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Calreticulin associates with stress proteins: Implications for chaperone function during heat stress


, : Calreticulin associates with stress proteins: Implications for chaperone function during heat stress. Journal of Cellular Biochemistry 69(1): 30-43

Acute heat stress leads to the glycosylation of a "prompt" stress glycoprotein, P-SG67/64, identified as calreticulin. In the present study, we used immunoprecipitation to investigate the interactions of P-SG/calreticulin with other proteins during cellular recovery from heat stress. In heat-stressed CHO and M21 cells, both glycosylated and unglycosylated P-SGs interact with HSP90, GRP94, GRP78, and the other prompt stress glycoprotein, P-SG50, in an ATP-independent manner. Specificity of HSP-P-SG interactions was determined by chemical cross-linking with the homo-bifunctional agent DSP (3,3'-dithiobis[succinimidyl propionate]). Characterization of the cross-linked complexes involving calreticulin and heat shock proteins (HSPs) showed an average mass of 400-600 kDa by gel filtration chromatography. Overall, the consistent association of glycosylated and unglycosylated calreticulin with P-SG50 and unglycosylated HSPs suggests that P-SG/calreticulin is an active member of the cast of glycone/aglycone chaperones that cooperate to achieve cellular recovery from acute heat stress.

US$19.90

PMID: 9513044

DOI: 10.1002/(sici)1097-4644(19980401)69:1<30::aid-jcb4>3.0.co;2-w


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Hill, H.J., 1958: The present status of therapy in animal infertility. Treatment of subfertility in male animals was discouraging because of lack of basic knowledge of reproductive processes. Although some types of infertility respond to endocrine or nutritive therapy, the incidence of failure shows the need for more...

Roka, L.; Hartwig, H.; Barth, D., 1985: Serum proteins of helminth-infected roe deer. Comparison between paper and immunoelectrophoresis. Six roe deer were given single or repeated doses of Chabertia, Ostertagia, Haemonchus, Trichostrongylus, and Dictyocaulus viviparus. Graphs are presented of the monthly changes in the alpha, beta and gamma globulins. These changes are discussed in...

Calhoon, T.B.; Gadsden, R.H.; Ham, C.H., 1963: The influence of histamine on the electrolyte and water content of subcutaneous connective tissue. Abstract. Direct application of histamine to connective tissue in vitro caused the same effects, with respect to electrolyte and water contents and net electrostatic charge, as those which are usually attributed to changes of capillary parameters...

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Azizi, E.; Semach, D.; Schewach-Millet, M.; Avni, I.; Ben-Haim, O.; Treister, G.; Blumenthal, M.; Belkin, M., 1984: Topical treatment of periocular basal cell epithelioma with solcoderm. 25 patients with 29 histologically confirmed periocular basal cell epitheliomas were treated by topical application of Solcoderm, a solution of organic and inorganic acids and ions. The cure rate of the 29 lesions, which were followed for a mean p...

Willey, L.A.; Zaleski, A., 1955: Lucerne strains. A discussion is given of the results of (1) classification work on strains under conditions in England (cf. PBA, XXIV, Abst. 2094), (2) an analysis of the leaf-stem ratio in different types on the basis of green and dry weights (cf. PBA, XXV, Abst...

Jones, I.M., 1962: Why the pool? Some reflections upon the remuneration of family doctors. British Medical Journal 1(5291): 1540-1546

Zhou, M.; Peterson, C., L.; Lu, Y.-Bin; Nadler, J.V.ctor, 1995: Release of glutamate and aspartate from CA1 synaptosomes: Selective modulation of aspartate release by ionotropic glutamate receptor ligands. Synaptosomes prepared from area CA1 of the rat hippocampus were used to determine (a) whether Schaffer collateral-commissural-ipsilateral associational terminals release both aspartate and glutamate in a Ca(2+)-dependent manner when reuptake of re...

Okamoto, T.; de Russo, M.C., 1973: Wound healing following tooth extraction. Histochemical study in rats. Revista Da Faculdade de Odontologia de Aracatuba 2(2): 153-169

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