+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Canine leishmaniasis: identification of asymptomatic carriers by polymerase chain reaction and immunoblotting

, : Canine leishmaniasis: identification of asymptomatic carriers by polymerase chain reaction and immunoblotting. American Journal of Tropical Medicine and Hygiene 55(3): 273-277

A major limitation to the study of the epidemiology of canine visceral leishmaniasis is the inability to identify and count asymptomatic carriers because classic diagnostic tests are insufficiently sensitive. We investigated the capacity of the polymerase chain reaction (PCR) to detect the parasite and immunoblotting to detect specific antibodies in samples from dogs living in an endemic area without any symptoms of leishmaniasis. Results of classic serologic tests (immunofluorescence and enzyme-linked immunosorbent assay) were negative. Two independent PCR assays detected the parasite in skin and conjunctival samples from 80% of the dogs. We found specific antibodies by immunoblot in 66% and 56% of the dogs had both specific antibodies and parasite DNA. As controls, samples from dogs with clinical manifestations of the disease before and after treatment were assayed. The frequency of positive PCR samples decreased after treatment, and although antibody levels decreased with cure of the disease, they remained detectable by immunoblot. Results showed that most of the dogs living in an endemic area had been exposed to Leishmania. Both PCR and immunoblot are sensitive enough to detect asyptomatic infection and could be valuable tools for studies monitoring the transmission of the disease and vaccination trials.


PMID: 8842114

Other references

Chassard, D.; Geneteau, A.; Gualano, V.; Brault, M., 2007: Bioequivalence study of two Ibuprofen formulations administered intravenously in healthy male volunteers. To compare the systemic bioavailability of two ibuprofen formulations, Pedea((R)) (ibuprofen intravenous [IV] formulation) and Imbun((R)) (ibuprofen intramuscular [IM] formulation) in 18 healthy male volunteers. Each subject received a 5 mg/kg dos...

Angelico, M.; Palmieri, G.; D.P.olo, D.; Torri, E.; Iaria, G.; Mensi, S.; Petrolati, A.; Anselmo, A.; Tisone, G., 2000: Early steatosis and past HBV infection predict fibrosis progression in liver transplant recipients with recurrent HCV infection. Gastroenterology 118(4 Suppl 2 Part 1): AASLD A995, April

Lin DongKang; Hou AnZu; M.Q.ngHai; L.Y.Lan; Wang ChengZhang; Chen HongKe; Zhao ZhiJie, 1995: The preventive effects of QRS antidote on Quercus poisoning in cattle. QRS is an antidotal formula containing ferrous sulfate which was developed to prevent Quercus poisoning in cattle. In field trials, oral administration of QRS antidote prevented Quercus poisoning in cattle in early spring, and it was better than o...

Tom W M.; Montgomery M.R., 1980: Bleomycin toxicity alterations in oxidative metabolism in lung and liver microsomal fractions. Separate groups of male rats received low doses (5 U) or high doses (15 U) of [the antineoplastic drug] bleomycin i.p. twice weekly for 1.5, 3 or 6 wk. The susceptibility of tissue lipid to peroxidation and the activities of mixed function oxidati...

Dias, E.; Laranja, F.S., 1949: Chagas' disease and its control. Proceedings 4th Intern Congr Trop Med Malaria Washington 1948. 1948 ; 2: 1159-1167

Hassay, K.A., 1995: Effective management of urinary discomfort. Urinary discomfort is the second most common physical complaint affecting women. Although urinary discomfort is commonly a result of inflammation due to bacterial invasion, there are also nonbacterial causes. The development of antimicrobial resis...

Gupta, N.; Mittal, S.; Misra, R., 2011: Inflammatory pseudotumor of uterus: an unusual pelvic mass. European Journal of Obstetrics, Gynecology, and Reproductive Biology 156(1): 118-119

Beneden, E.Van., 1869: The development of the ovum of various orders of Crustacea is the subject of several papers by ED. V. The development of the ovum of various orders of Crustacea is the subject of several papers by ED. VAN BENEDEN, which are noticed above. It is very remarkable that there is a difference between the freshwater and marine species of Gammarus: in the...

Tomaszewska, A.; Zelaźniewicz, A., 2015: Morphology and morphometry of the meningo-orbital foramen as a result of plastic responses to the ambient temperature and its clinical relevance. The meningo-orbital foramen (FMO) is an osteal opening, containing vessels providing an accessory blood supply to the orbit, situated close to the superior orbital fissure. Recent studies show FMO to be ubiquitous, with localization and occurrence...

Alcantara H.; Goncalves P.W., 2000: Creationism and religious intolerance; how can we work in earth science classes? A research on the geography classes in Campinas, Brazil. International Geological Congress, Abstracts = Congres Geologique International, Resumes 31: (unpaginated