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Catalytic characteristics of catalase in microemulsions of surfactants in octane

, : Catalytic characteristics of catalase in microemulsions of surfactants in octane. Biochemistry (Moscow) 61(9): 1182-1192

The rate constants of H-2O-2 decomposition, interaction of catalase complex I with H-2O-2, and the effective rate constants of catalase inactivation during the enzymatic catalysis (k-in) were determined by transformation of the complete kinetic curves of H-2O-2 decomposition by catalase in reversed micelles of Aerosol OT (AOT)-3 in octane and aqueous solution. Effects of the degree of hydration of reversed AOT micelles and concentrations of AOT, H-2O-2, and catalase itself in the micellar systems on each of the three kinetic constants were studied in detail. Optimal conditions were found which provide for high catalase stability and activity in micellar systems as compared with aqueous solutions. The stability of catalase is significantly enhanced (k-in decreases) in the presence of reduced glutathione and ethanol in AOT micelles. Partial dissociation of catalase to subunits in reversed AOT micelles was demonstrated by their peroxidase activity in cumene hydroperoxide-dependent oxidation of tetramethylbenzidine. Catalase dissociation to monomers decreases significantly in mixed micelles composed of AOT, Triton X-45, Triton X-100, or Tween-85 and octanol.


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