geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Catalytic characteristics of catalase in microemulsions of surfactants in octane


, : Catalytic characteristics of catalase in microemulsions of surfactants in octane. Biochemistry (Moscow) 61(9): 1182-1192

The rate constants of H-2O-2 decomposition, interaction of catalase complex I with H-2O-2, and the effective rate constants of catalase inactivation during the enzymatic catalysis (k-in) were determined by transformation of the complete kinetic curves of H-2O-2 decomposition by catalase in reversed micelles of Aerosol OT (AOT)-3 in octane and aqueous solution. Effects of the degree of hydration of reversed AOT micelles and concentrations of AOT, H-2O-2, and catalase itself in the micellar systems on each of the three kinetic constants were studied in detail. Optimal conditions were found which provide for high catalase stability and activity in micellar systems as compared with aqueous solutions. The stability of catalase is significantly enhanced (k-in decreases) in the presence of reduced glutathione and ethanol in AOT micelles. Partial dissociation of catalase to subunits in reversed AOT micelles was demonstrated by their peroxidase activity in cumene hydroperoxide-dependent oxidation of tetramethylbenzidine. Catalase dissociation to monomers decreases significantly in mixed micelles composed of AOT, Triton X-45, Triton X-100, or Tween-85 and octanol.

US$29.90


Other references

Skopnik, H.; Schüttler, A.; Döhmen, H.; Geron, M.; Heimann, G., 1989: Glucose homeostasis in mucoviscidosis. In patients with cystic fibrosis (CF) of the pancreas an endocrine imbalance especially of insulin secretion due to progressive structural abnormalities of the pancreas must be expected. 30-75 percent of CF-patient exhibit impaired oral glucose to...

Effert S., 1969: Emergency situations due to cardiac rhythm disturbances in internal medicine. Internist: 224-234

Marmont A.M., 1990: Selective metaphasic arrest of erythroid precursors in hodgkins disease treatment with erythropoietin and mopp. Experimental Hematology (Charlottesville) 18(6): 570

Renoux G.; Renoux M., 1975: Effects of levamisole on immunologic induction of thymus derived lymphocytes. Annales d'Immunologie (Paris) 126C(1): 91-92

Kovalenko, V.; Voronina, A.; Okhrimenko, V., 2001: Caffeine potentiation of acetaminophen hepatotoxicity. Toxicology Letters (Shannon) 123(Supplement 1): 125, September 1st

Manilal K.S.; Sivarajan V.V., 1976: Flowering plants of the calicut university campus. Manilal, K S And V V Sivarajan Flowering Plants Of The Calicut University Campus 24p Calicut University: Calicut, Kerala, India 1-24

Abe, H.; Machida, T.; Yada, K.; Tsuru, M., 1968: Critical zone in acute increase of intracranial pressure. No to Shinkei 20(1): 5-10

Murray Christopher J.; Chien Y.J.; Thorne Paul D., 2003: Geostatistical analysis of historical groundwater concentration data for calibration of flow and transport models. Large amounts of historical data are available on the concentration of selected contaminants in groundwater at the Hanford Site, and this data can be used to evaluate fate and transport model performance used for decision making at the Site, inclu...

Sakata, M., 1996: Expression of MAGE gene family in lung cancers. MAGE gene family encodes tumor-specific antigens recognized on HLA-A1 by cytotoxic T lymphocytes. We have investigated the mRNA expression of MAGE gene family in lung cancers using MAGE gene specific primers, reverse transcription-polymerase chain...

Beenken S.; Karsenty G.; Lozano G., 1990: Base pair substitution inhibits protein binding to intron 4 of the p53 gene. Proceedings of the American Association for Cancer Research Annual Meeting 31: 323