geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Changes in nerve growth factor immunoreactivity following entorhinal cortex lesions: possible molecular mechanism regulating cholinergic sprouting


, : Changes in nerve growth factor immunoreactivity following entorhinal cortex lesions: possible molecular mechanism regulating cholinergic sprouting. Journal of Comparative Neurology 345(3): 409-418

To assess the possible role of trophic factors in lesion-induced plasticity, we have used a sensitive immunohistochemical technique to evaluate changes in nerve growth factor (NGF) staining in the hippocampal formation 3, 8, 16, and 30 days following entorhinal cortex lesions. Our results indicate that a band of NGF immunoreactivity appears in the outer molecular layer of the ipsilateral dentate gyrus following entorhinal ablation. The distribution of the NGF-immunoreactive band exactly coincides with the distribution of sprouting cholinergic terminals revealed by acetylcholinesterase histochemistry or NGF-receptor immunostaining. Increased NGF-immunoreactivity is detectable at 3 days postlesion, is most intense at 8 days, and decreases to near control levels by 30 days. Lesion-induced increases in NGF immunostaining also occur in animals in which septohippocampal fibers had been removed by prior destruction of the fimbria-fornix. Increases in NGF-immunoreactivity, however, are substantially reduced in animals receiving intraventricular injections of colchicine, which presumably blocks NGF release. These results indicate that 1) increases in NGF immunostaining, which occur following entorhinal lesions, precede any changes in cholinergic sprouting parameters and are greatest during the period of maximal cholinergic sprouting; 2) increased NGF-immunoreactivity is not due to NGF binding by septohippocampal fibers; and 3) increased NGF-immunoreactivity appears to depend on the release of NGF by neurons that produce it. We hypothesize that, following entorhinal lesions, NGF immunostaining within the hippocampal formation may represent NGF "anchored" within the tissue and that NGF accumulation by such a mechanism may direct the sprouting response of NGF-sensitive cholinergic neurons.

US$19.90

PMID: 7929909

DOI: 10.1002/cne.903450307


Other references

Nalepa, C.A.; L.L.; L.W.nHua; Lazell, J., 2001: Rediscovery of the wood-eating cockroach Cryptocercus primarius (Dictyoptera: Cryptocercidae) in China, with notes on ecology and distribution. The wood-eating cockroach, Cryptocercus primarius, previously recorded only from Sichuan Province, is reported from 2 areas in the Hengduan Mountains of northwestern Yunnan Province, China. Survival of this species, critical to systematic and evol...

Gaillot, J.; Bieder, A.; Ghaye Roquet, J.; L.L.boux, A.; Mazeau, L., 1975: A cyamepromazine-trifluoperazine combination: changes in metabolism during chronic administration in the rat. Therapie 30(2): 267-276

McCormick, S.P.; Fellowes, A.P.; Walmsley, T.A.; George, P.M., 1992: Apolipoprotein B-32: a new truncated mutant of human apolipoprotein B capable of forming particles in the low density lipoprotein range. We have identified a new species of apolipoprotein (apo) B in an individual with heterozygous hypobetalipoproteinemia. The new apo B (apo B-32) is the result of a single point mutation (1450 Gln----Stop) in the apo B gene that prevents full length...

Thakoersing, V.S.; Ponec, M.; Bouwstra, J.A., 2010: Generation of human skin equivalents under submerged conditions-mimicking the in utero environment. In this study we generated human skin equivalents (HSEs) under submerged conditions mimicking the aqueous in utero environment and investigated the morphology and differentiation process of the formed epidermis. Further, the skin barrier, which re...

Sullivan, P.A.; Fisher, P.S., 1995: Challenges in a multidisciplinary head and neck oncology program. Cancer Practice 3(4): 258-260

Vadapalli, V.R.K.; Zvimba, J.N.; Mulopo, J.; Motaung, S., 2013: Sulphate removal from sodium sulphate-rich brine and recovery of barium as a barium salt mixture. Sulphate removal from sodium sulphate-rich brine using barium hydroxide and recovery of the barium salts has been investigated. The sodium sulphate-rich brine treated with different dosages of barium hydroxide to precipitate barium sulphate showed...

Frau, G., 1971: Coronary diseases and beta-blocking agents. Annales de Cardiologie et D'angeiologie 20(1): 59-64

Anonymous, 1976: Thrombus formation around an endocardial pacemaker electrode: a scanning electron microscopic study and its relationship to abnormal threshold elevation. The author experienced an abnormal increase in stimulation threshold in one patient to whom a permanent endocardial electrode was implanted according to the temporary-permanent technique, but could safely exchange the electrode at the fifth postop...

Lardeux, F.; Rivière, F.; Séchan, Y.; Loncke, S., 2003: Control of the Aedes vectors of the dengue viruses and Wuchereria bancrofti: the French Polynesian experience. In most of the 130 islands of French Polynesia, the stenotopic mosquitoes Aedes aegypti (the main local vector for the viruses causing dengue) and Aedes polynesiensis (the main local vector of Wuchereria bancrofti) share many breeding sites in wat...

Worsley, S.D.; Ponder, B.A.; Davies, B.R., 1997: Overexpression of cyclin D1 in epithelial ovarian cancers. Amplification and overexpression of the cell cycle-related gene cyclin D1 have been demonstrated in several human malignancies and have been shown to be directly oncogenic in breast epithelium and lymphocytes. Overexpression of the gene can occur...