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Characterization of carcinoma cells ex vivo on LASER-excited fluoroscopy using a newly synthesized porphyrin derivative PH1008


, : Characterization of carcinoma cells ex vivo on LASER-excited fluoroscopy using a newly synthesized porphyrin derivative PH1008. Yamagata Medical Journal 15(2): 65-76

This study was designed to elucidate fluorescent properties of newly synthesized porphyrin derivative, PH1008, and its cytological effects and to assess the clinical feasibility for the field of mass-screening of lung cancer cell ex vivo. Ten mM solution of PH1008 excited with He-Cd laser emitted fluorescence at 700 nm. In a higher concentration (100 mM PH1008), another peak was noted at 650 nm which fluorescence easily merged in 700 nm after sonication. This finding suggested the existence of PH1008 as a dimmer at a concentration of 100 mM. Adding fetal calf serum to 100 mM PH1008 solution, two peaks at 650 nm and 700 nm shifted to 630 nm and 680 nm, respectively. When cultured cancer cells (Hela cell) were incubated with 0.5 mM PH1008, increasing intensities of fluorescence with peaks at 630 nm and 680 nm were detected in a time dependent manner. Cytotoxicity of PH1008 on Hela was observed in dose- and time-dependent manner. Subcellular distribution and its fluorescent spectra of PH1008 intensity was determined on single cell using microscopical fluorescent spectroscopy. The fluorescence was detected mainly at the perinuclear area in A-549 cell and the main peak moved from 680 nm at 20 min to 630 nm at 120 min, suggesting that A-549 cells initially accumulated PH1008 as a monomer and then PH1008 formed a dimer with a prolongation of incubation time. The characteristic peaks of emitted fluorescence at 630 nm and 680 nm were also observed in surgically resected cancer cells whereas no or minimum fluorescence was observed in normal lung cells. We concluded that our laser-excited fluorescent system could be applicable to the mass screening of the lung cancer cells ex vivo.

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