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Characterization of native and recombinant bone sialoprotein: delineation of the mineral-binding and cell adhesion domains and structural analysis of the RGD domain


, : Characterization of native and recombinant bone sialoprotein: delineation of the mineral-binding and cell adhesion domains and structural analysis of the RGD domain. Journal of Bone and Mineral Research 12(8): 1210-1222

Bone sialoprotein is a small, sulfated, and phosphorylated integrin-binding glycoprotein apparently found only in tissues that eventually mineralize. Nondenatured bone sialoprotein (BSP) purified from rat osteosarcoma cell line (UMR 106-01 BSP) culture media is shown to have a hydroxyapatite Kd approximately 2.6 x 10(-9) M, perhaps the strongest affinity for this mineral of any of the matrix proteins. Both native BSP and a 47 kD fragment of UMR-BSP (Fragment 1 approximately 133A- approximately 265Y) are more potent inhibitors of seeded hydroxyapatite crystal growth than recombinant human BSP fragments lacking post-translational modifications. The recombinant proteins, however, do show reproducible inhibitory activity, suggesting that at least some of the strong mineral-binding properties are encoded directly within the protein sequence itself. BSP facilitates the adhesion of several cell types through its integrin binding (RGD) tripeptide sequence. Nuclear magnetic resonance (NMR) analysis of a 15N-enriched 59 amino acid recombinant domain containing the RGD tripeptide shows that the structure of this isolated domain is highly flexible with or without 5 mM calcium. Previous work has also shown that an endogenous fragment of UMR-BSP (Fragment 1) supports cell adhesion in the absence of the RGD sequence. In this report, non-RGD cell adhesion sites are localized within conserved amino- and carboxy-terminal tyrosine-rich domains of recombinant human BSP. Given the proximity of the latter non-RGD cell adhesion site to the RGD tripeptide, a model of BSP-receptor interactions is presented.

US$19.90

PMID: 9258751

DOI: 10.1359/jbmr.1997.12.8.1210


Other references

Taussig, B.L.; Hendin, A., 1955: Black widow spider poisoning. Missouri Medicine 52(9): 714-716

Kozakiewicz, J.; Szarmach, H.; Malinowska, H., 1966: Histological studies on the development of skin neoplasms induced by methylcholanthrene in white mice. Polish Medical Journal 5(6): 1370-1378

Gurevich M.I., 1988: Optimal discrete filter for data processing of marine gravimetric measurements. Pages 47-51 1988

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Salomon, J.C.; Lynch, N., 1977: Treatment of solid tumours by intratumoral injection of immunostimulants. Intra-lesional injections of immunostimulants--BCG or Corynebacterium parpum--induced a local cure of the McFiFj2(S) tumour and a remote therapeutic effect. When applied to rats multigrafted with the McFiFi2(S) tumour, local and distant cures were...

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Huffaker, R.C.; Wallace, A., 1959: Sodium absorption by different plant species at different potassium levels. Studies were designed to test the relative importance of plant species differences and K and Na levels in nutrient solutions on influencing Na accumulation by some plant species. Corn, soybean, and radish plants were grown for 48 hours in solution...

Buhler M.I.; Petrino T.; Legname A.H., 1987: Sperm nuclear transformation and aster formation related to metabolic behaviour in amphibian eggs. Bufo arenarum oocyte maturation is related to important modifications in the metabolism of carbohydrates. Such changes involve a different relative participation of the main oxidative routes and are under the influence of seasonal variations of th...