geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Characterization of recombinant CD45 cytoplasmic domain proteins. Evidence for intramolecular and intermolecular interactions


, : Characterization of recombinant CD45 cytoplasmic domain proteins. Evidence for intramolecular and intermolecular interactions. Journal of Biological Chemistry 273(28): 17839-17845

CD45 is a transmembrane two-domain tyrosine phosphatase required for efficient signal transduction initiated by lymphocyte antigen receptors. As with most transmembrane two-domain phosphatases, the role of the second phosphatase domain is unclear. In this study, recombinant CD45 cytoplasmic domain proteins purified from bacteria were used to evaluate the function of the individual phosphatase domains. A recombinant protein expressing the membrane-proximal region, first phosphatase domain, and spacer region of CD45 (rD1) was catalytically active and found to exist primarily as a dimer. In contrast to this, a recombinant protein expressing the spacer region, the second phosphatase domain and the carboxy tail of CD45 (rD2) existed as a monomer and had no catalytic activity against any of the substrates tested. Comparison of rD1 with the recombinant protein expressing the entire cytoplasmic domain of CD45 (rD1/D2) indicated that rD1/D2 was 2-3-fold more catalytically active, was more thermostable, and existed primarily as a monomer. Limited trypsin digestion of rD1/D2 provided evidence for a noncovalent association between an N-terminal 27-kDa fragment and a C-terminal 53-kDa fragment, suggesting an intramolecular interaction. Furthermore, rD1 was found to specifically associate with rD2 in an in vitro binding assay. Taken together, these data provide evidence for an intramolecular interaction occurring in the cytoplasmic domain of CD45. In the absence of the C-terminal region containing the second phosphatase domain, intermolecular interactions occur, resulting in dimer formation.

US$19.90

PMID: 9651387

DOI: 10.1074/jbc.273.28.17839


Other references

Gerasimova L.I.; Yurasov I.I.; Skobelina G.S., 1980: Dynamics of basal metabolism in patients with burns. The study of dynamics of the factual basal metabolism (FBM) over 35 days of treatment in 77 patients with burns covering 10-50% of the skin surface showed a dependence of changes in FBM on burn area, patient's age and complications (pneumonia...

Bauch Dorothea; Darling Kate F.; Simstich Johannes; Bauch Henning A.; Erlenkeuser Helmut; Kroon Dick, 2002: Genetic evidence reveals species dependent delta (super 18) O shift in Neogloboquadrina pachyderma (dextral) between glacial and interglacial times. Eos, Transactions, American Geophysical Union 83(47, Suppl

Hyman, D.A.; Williamson, J.V., 1989: Fraud and abuse. Setting the limits on physicians' entrepreneurship. New England Journal of Medicine 320(19): 1275-1278

Bonanni, P.; Gershon, A.; Gershon, M.; Kulcsár, A.; Papaevangelou, V.; Rentier, B.; Sadzot-Delvaux, C.; Usonis, V.; Vesikari, T.; Weil-Olivier, C.; de Winter, P.; Wutzler, P., 2014: Primary versus secondary failure after varicella vaccination: implications for interval between 2 doses. Two-dose varicella vaccination is recommended for optimal control of varicella in populations with high (>90%) 1-dose coverage. Optimal timing of the second dose may depend on whether breakthrough varicella results from primary vaccine failure...

Sulaiman, M., 1941: The influence of light on nitrogen fixation in Dacca soil. Jour Indian Chem Soc 18(1): 40-42

Hilali, N.; Kocarslan, S.; Vural, M.; Incebiyik, A.; Camuzcuoglu, A.; Camuzcuoglu, H., 2015: Ki-67 proliferation index in patients with placenta previa percreta in the third trimester. The purpose of this study was to investigate proliferative capacity of placenta previa percreta in the third trimester via evaluating Ki-67 proliferating index. The paraffin blocks of placental tissues, which were obtained from the patients who un...

Geggel, H.S., 2006: Arcuate relaxing incisions guided by corneal topography for postkeratoplasty astigmatism: vector and topographic analysis. To determine if using corneal topography for planning arcuate relaxing incision placement for postkeratoplasty astigmatism improves clinical results. Twenty-six eyes with high levels (> 5 diopters [D]) of postkeratoplasty astigmatism were studi...

Azuma, J.; Tanaka, F.; Koshijima, T., 1984: Microwave irradiation of lignocellulosic materials. I. Enzymatic susceptibility of microwave-irradiated woody plants. Samples of Pinus densiflora, Fagus crenata and Phyllostachys edulis were subjected to microwave irradiation in the presence of water followed by enzymatic saccharification. The enzymatic susceptibility of the samples was markedly enhanced by micro...

Bhuiyah I.M.; Islam N.; Begum A.; Karim M.A., 1990: Biology of the rice weevil sitophilus oryzae linnaeus. The rice weevil Sitophilus oryzae (Linnaeus) is the most destructive pest of stored rice. The incubation period, larval and pupal periods were 5-6, 16-20 and 8-9 days, respectively. The adult longevity of male was 114-115 days and that of female w...

Ozzello L.; Derosa C.; Habif D.V.; Greene G.L., 1991: An immunohistochemical evaluation of progesterone receptor in frozen sections paraffin sections and cytologic imprints of breast carcinomas. Two monoclonal antibodies to progesterone receptor (PR), JZB39 and KD68, were used for the immunocytochemical visualization of PR in different kinds of breast cancer specimens including (1) cryostat sections of tumors frozen at -80.degree. C; (2)...