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Characterization of the role of individual protein binding motifs within the hepatitis B virus enhancer I on X promoter activity using linker scanning mutagenesis


, : Characterization of the role of individual protein binding motifs within the hepatitis B virus enhancer I on X promoter activity using linker scanning mutagenesis. Virology 193(2): 653-660

A combination of linker scanning mutagenesis and deletional analyses has been used to determine the role of individual DNA:protein binding sites on expression from the hepatitis B virus (HBV) enhancer I-X promoter (map position (mp) 1042-1354, HBV adw2). Linker scanning mutation of the EF-C site caused a 67.5% drop in X promoter activity in HuH7 cells, but had no effect in HepG2 or HepSK cells. Mutation of the E element resulted in an approximately 50% reduction in X promoter activity in HuH7, HepG2, and HepSK cells. Deletional analysis showed that sequences upstream of the EF-C site (mp 1163) were required for full X promoter activity and implicated the NF-1a site as being sufficient for basal X promoter activity. However, PCR-directed linker scanning mutation of the NF-1a site did not cause a reduction in X promoter activity, indicating that this site was not an essential component of the X promoter. Taken together, these results indicated that multiple, partially redundant protein:DNA interactions in the enhancer 1 are essential for full X promoter activity. The lack of an essential basal promoter element supports the suggestion that the two separate HBV enhancer elements (enhI and enhII) were created by integration of the X gene into a primordial enhancer element.

US$19.90

PMID: 8384750

DOI: 10.1006/viro.1993.1173


Other references

Barcelo D.; Petrovic M.; Eljarrat E., 2003: The discovery of emerging contaminants for soil and water protection. Tuebinger Geowissenschaftliche Arbeiten Reihe C, Hydro-, Ingenieur- Umweltgeologie

Dokov, V.K., 1961: L'eosinophilie pulmonaire chez les amphibiens et les reptiles. Folia Biologica Krakow, 9: 151-158

Herrick, T.W.; Yeager, H., 1989: Home oxygen therapy. Home oxygen therapy is clearly beneficial for the chronically hypoxemic patient. Compressed gas, liquid oxygen and oxygen concentrators are the primary oxygen sources for home use. A number of oxygen-conserving devices are now available and may im...

Babu, S.C.; Shah, P.M.; Moggio, R.A.; Choudhury, M., 1993: Involvement of inferior vena cava in renal cancer Guidelines for operative management. Journal of Vascular Surgery 17(2): 441

Singh, I.B.; Singh, P., 1976: Path analysis of pearl millet. Path analysis of data from 72 diverse lines of Pennisetum typhoides grown in a single block with two replications and between-row spacings of 20 and 50 cm, respectively, revealed that days to flower, total tiller number and number of leaves showed...

Slatin, S.; Jakes, K.; Qiu, X.Q.ng; Finkelstein, A., 1994: Site-directed biotinylation used to identify a voltage-dependent conformational change in the colicin Ia channel. Biophysical Journal 66(2 PART 2): A439

Platonov, A.V.; Kochereshko, V.P.; Kats, V.N.; Tsyrlin, G.E.; Buravlev, A.D.; Samsonenko, Yu. B.; Besombes, L.; Mariette, H., 2013: Optical properties of individual GaAs quantum dots embedded into AlGaAs nanowires. The photoluminescence (PL) spectra of semiconductor structures, namely, Al<sub>0.3</sub>Ga<sub>0.7</sub>As-based quasi-one-dimensional cylindrical nanowires (nanowhiskers), are measured. The diameter of a typical nanowire i...

Brosseau, B.L., 1971: Who says we'll have to relearn metric?. Canadian Hospital 48(6): 6-7

Dragnev D.; Zhivkova I., 1976: Serum alkaline phosphatase activity during the latter half of embryogenesis in pig fetuses. Zhivotnov"dni Nauki 13(4): 47-50

Kumar D.; Saxena Y., 1991: Effects of an anticoagulant rodenticide brodifacoum on the haematological aspects of albino mice. Haematological studies were carried out in the laboratory on the Swiss albino mice after Brodifacoum treatment. Several parameters of control and poisoned mice of both the sexes were recorded. The results reveal that the haemoglobin, haematocrit a...