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Characterizing submicron vesicles with wavelength-resolved fluorescence in flow cytometry

, : Characterizing submicron vesicles with wavelength-resolved fluorescence in flow cytometry. Cytometry 25(2): 144-155

Individual synthetic vesicles 0.1-1.0 mu-M in diameter are sized by using single-particle fluorescence emission spectra obtained in a custom sheath flow cell with an imaging spectrograph and a charge-coupled device. Data are acquired at 1 Hz, with limits of detection (3-sigma) less than 6.0 times 10-3 and 1.0 times 10-4 molecules of free sulforhodamine 101 and fluorescein, respectively, and with a spectral range for fluorescence emission collection from 350 to 800 nm (0.45 nm/pixel resolution). The system is used for small-particle population analysis by analyzing a suspension of submicron, unilamellar synthetic vesicles prepared by standard procedures with phosphatidylserine and Texas red- or fluorescein head-group-conjugated dihydropalmitoylphosphatidylethanolamine. The submicron particles are individually identified, sized, and discriminated based on single-particle fluorescence emission spectra. Excellent agreement is found between fluorescence sizing data and transmission electron microscopic measurements.


PMID: 8891444

DOI: 10.1002/(SICI)1097-0320(19961001)25:2<144::AID-CYTO3>3.0.CO;2-H

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Antinoro-Polizzi, J., 1971: Societal types and developing strategies. Modernization, change or economic development in society is a very complex phenomenon involving the operation of several factors or variables over time. Agents of change have often narrowly misinterpreted their nature. In dealing with ' commu...

Kapronczay, K.; Hapsburg family; von Sonnenfels, J., 2001: Cameralism and medical policy of the enlightened absolutism. Lege Artis Medicinae 8(5): 384-388

Penny, C.D.; Hahn, C.; Macrae, A.; Mayhew, J., 2005: Wobbler syndrome in pedigree rams. This correspondence describes the clinical signs and results of a clinical investigation using contrast myelography and computed tomography in Wobbler syndrome or cervical vertebral malformation cases in rams.

Tedoradze, S.G.; Tbilelashvili, N.V., 1972: Mode of inheritance in hybrids obtained by crossing Canadian with Georgian wheats. The Georgian wheats were improved by hybridization with Canadian varieties of the intensive type. In growth period the hybrids approached winter varieties and some hybrids had growth periods seven days shorter than their parents. In winter hardine...

Island M.; Naider F.; Becker J.M., 1986: Micromolar concentrations of amino acids induce dipeptide uptake in saccharomyces cerevisiae. Abstracts of the Annual Meeting of the American Society for Microbiology 86: 227

Kumar Ghosh, S., 2015: Resident doctors: keystone of anatomy teaching. Clinical Teacher 11(6): 461-462

Miley, C.; Brann, M.; Amburgey, J.; Soper, D.; Wos, J.; Wang, Y.; Hartke, J.; De, D.; Delong, M.A., 1998: Activity and receptor selectivity of 17-phenyl-trinor PGE2, 9-methylene-9-deoxy-PGE2 and 9-substituted analogs at cloned human EP1 and EP3 receptors. Abstracts of Papers American Chemical Society 215(1-2): MEDI 50B

Mutimer, D.; Tang, H.; Dragon, B.; Gutekunst, K.; Barmat, S.; Ahmed, M.; O'donnell, K.; Shaw, J.; Elias, E., 1997: Antiviral treatment of hepatitis B virus infected patients Evaluation of response using a sensitive quantitative polymerase chain reaction assay. Gastroenterology 112(4 SUPPL ): A1341

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Kadobayashi I.; Kira Y.; Toyoshima A.; Nishijima H., 1984: Auditory middle latency responses in relation to electrode combinations and stimulus conditions. Auditory middle latency responses were recorded from healthy right-handed subjects. Acoustic clicks at a rate of 10/s were presented to the subjects via headphones. Using the electrode combination unilateral mastoid-nose tip, responses characteriz...