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Chemical analysis of multiple sclerosis lesions by FT-IR microspectroscopy

, : Chemical analysis of multiple sclerosis lesions by FT-IR microspectroscopy. Free Radical Biology & Medicine. 25(1): 33-41, Y 1

Fourier transform infrared microspectroscopy can be used to collect infrared spectra from microscopic regions of tissue sections. If spectra are collected along a grid pattern, then maps of chemical functional groups can be produced and correlated to tissue histopathology. In the present study, white matter from multiple sclerosis and control brains were examined. Mapping experiments were designed such that 17 spectra were collected at 200 microm intervals along a line that was partially or wholly within a multiple sclerosis lesion site or within a representative white matter region of control tissue. Data analysis was based on earlier in vitro studies which found that the carbonyl at 1740 cm(-1) increases when lipids become oxidized (Free Rad. Biol. Med. 16:591-601, 1994), and the amide I peak at approximately 1660 cm(-1) broadens when proteins become oxidized (FEBS Let. 362:165-170, 1995). The results indicated that the C=O to CH2 ratio (1740 cm(-1):1468 cm(-1)) was elevated at several collection points in lesion sites from multiple sclerosis brains compared to values from white matter of control brains. Inspection of the amide I peak at 1657 cm(-1) revealed that it was broadened towards 1652 cm(-1) in multiple sclerosis tissues but not control tissues. These results suggest that lipids and proteins could be oxidized at active multiple sclerosis lesion sites. The localization of these products to lesion sites supports a role for free radicals in the pathogenesis of multiple sclerosis.


PMID: 9655519

DOI: 10.1016/s0891-5849(98)00019-7

Other references

Burakhovich, M.S.; Gribanova, V.I.; Velichko, V.I.; Larina, M.V.; Iakovleva, V.I., 1976: Hygienic evaluation of the operating efficiency of the purification structures of the Kharkov Tractor Factory. Gigiena i Sanitariia: 97-98

Silbereben, Karl, 1951: The use of photoelectric colorimetry in the brewery laboratory. Lange's photoelectric colorimeter is descr. It has been used to measure wort and beer color and Indicator Time Test values. Silbereisen uses it for determining ammonium sulfate turbidity.

Hahn, P.P., 1970: Tooth mobility employing holding and supporting elements of bent wire. Cornell Veterinarian 60(4): 280-287

Bridger G.M.; Yang W.; Mckersie B.D.; Falk D.E., 1988: Relationship between paraquat and freezing tolerances in winter cereals. Plant Physiology (Rockville) 86(4 SUPPL): 52

Johnson, B.; Li, J.; Adhikari, J.; Edwards, M.R.; Zhang, H.; Schwarz, T.; Leung, D.W.; Basler, C.F.; Gross, M.L.; Amarasinghe, G.K., 2016: Dimerization Controls Marburg Virus VP24-dependent Modulation of Host Antioxidative Stress Responses. Marburg virus (MARV), a member of the Filoviridae family that also includes Ebola virus (EBOV), causes lethal hemorrhagic fever with case fatality rates that have exceeded 50% in some outbreaks. Within an infected cell, there are numerous host-vir...

Ho, P-Yu.; Wang, Y.; Yiu, S-Chun.; Yu, W-Hong.; Ho, C-Lam.; Huang, S., 2017: Starburst Triarylamine Donor-Based Metal-Free Photosensitizers for Photocatalytic Hydrogen Production from Water. Three metal-free molecular photosensitizers (S1-S3) featuring a starburst triarylamine donor moiety have been synthesized. They show attractive photocatalytic performance in visible light-driven H<sub>2</sub> production from water in t...

Kato, K., 1992: Efficacy and safety of amiodarone in treatment of refractory atrial and ventricular tachyarrhythmias. The efficacy and the safety of amiodarone therapy in 82 patients with recurrent tachyarrhythmias, refractory to conventional antiarrhythmics, were evaluated by a multicenter trial. Clinical episodes of ventricular fibrillation, sustained and non-s...

Krylova, N.A., 1967: Determining alpha-methylstyrene by the infrared spectroscopy method. Gigiena i Sanitariia 32(1): 74-75

Niemczyk, S.; Ludwicka, A.; Groniowski, M.; Lewandowski, Z.; Hasse, Z.; Wardyn, K.A., 1991: Nephrotoxicity of aminoglycosides. II. Preventive studies with oral administration of verapamil. The effect was studied of oral verapamil administration in two different doses on gentamicin nephrotoxicity. Three groups of Wistar male rats were studied: 1) treated with gentamicin 100 mg/kg b.w. subcutaneously, 2) treated with gentamicin s.c. a...

Miller C.; Pfister R., 1991: Organotin resistant microorganisms and their potential for controlling dreissena polymorpha zebra mussel. Abstracts of the General Meeting of the American Society for Microbiology 91: 321