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Chronic opioid antagonist administration upregulates mu opioid receptor binding without altering mu opioid receptor mRNA levels


, : Chronic opioid antagonist administration upregulates mu opioid receptor binding without altering mu opioid receptor mRNA levels. Molecular Brain Research. 33(2): 351-355

Chronic administration of opioid antagonists has been shown to increase radioligand binding to brain opioid receptors. The present study was conducted to determine whether chronic exposure to the opioid antagonist naltrexone would similarly increase mu opioid receptor gene expression as measured by mRNA levels. Male Sprague-Dawley rats were administered naltrexone, 7-8 mg/kg/day, or saline by osmotic minipumps for 7 days. As expected, the density of mu opioid receptor binding sites was significantly higher in the brains of animals treated chronically with naltrexone as compared with saline-treated control animals. However, mu opioid receptor mRNA content determined by a solution hybridization RNase protection assay was not significantly altered in any brain region investigated. These results indicate that the upregulation of mu opioid receptors as measured by radioligand binding is not accompanied by increased levels of mu receptor mRNA.

US$19.90

PMID: 8750897

DOI: 10.1016/0169-328x(95)00143-g


Other references

Lu, X.Y.; Ghasemzadeh, M.B.; Kalivas, P.W., 1998: The regional distribution and cellular localization of GABAB1 receptor messenger RNA in the rat brain. Society for Neuroscience Abstracts 24(1-2): 1587

Josifov, Mv, 1983: Distribution of many heteropteran species on the Balkan Peninsula. II. Acta zoologica bulgarica: (22) 3-8

Buniatian, A.A.; Kozhevnikov, V.A.; Trekova, N.A.; Flerov, E.V., 1982: Combined intravenous hypnoanalgesia without inhalation anesthetics. Anesteziologiia i Reanimatologiia: 5-9

Hoestlandt, H.; Rudelle, J., 1979: Examen electronique densitometrique d'opercules de perches (Perca fluviatilis L.) en vue de la determination de l'age des poissons. Comptes Rendus Hebdomadaires Des Seances De L'academie Des Sciences Serie D Sciences Naturelles: 2881: 117-118

Luo XiaoYong; Matsumoto, H., 2002: Susceptibility of a broad-leaved weed, Acanthospermum hispidum, to the grass herbicide fluazifop-butyl. Broadleaved plants are generally resistant to aryloxyphenoxypropionate (AOPP) and cyclohexanedione (CHD) herbicides. In laboratory experiments, however, we confirmed that A. hispidum, a Compositae weed, was susceptible to one of the AOPP herbicide...

Phillips, J.C., 1932: Eastern and western bred waterfowl at Wenham, Massachusetts, in the past thirty years. Auk: 440-444

Boussouar, I.; Chen, Q.; Chen, X.; Zhang, Y.; Zhang, F.; Tian, D.; White, H.S.; Li, H., 2017: Single Nanochannel Platform for Detecting Chiral Drugs. Chiral drugs play an essential role in medical and biochemical systems, and thus enantioselective analysis of chiral molecules has become a central focus in chemical, biological, medical, and pharmaceutical research. The design of chiral drug-dete...

Koseva D.; Koleva S.; Decheva R., 1979: Changes in free amino acid and nucleic acid contents in flower buds of the kazanlik rose at the time of abscission. The qualitative and quantitative composition of free amino acids and the nucleic acid content of normally developing and abscissing flower buds of the Kazanlik damascena rose were studied. The formation of a separating layer in the basis of the fl...

Almazan, R.R.; Rubio, R.P.; Agoramoorthy, G., 2005: Welfare evaluations of nonhuman animals in selected zoos in the Philippines. This study evaluated 3 zoos in the Philippines: the Wildlife Rescue Center and Mini Zoo, Manila Zoological and Botanical Garden, and Cavite Botanical and Zoological Park to determine the standards of nonhuman animal welfare. The study measured and...

Mcgavack, T.H.; Schwimmer, D., 1944: Problems in the treatment of cardiac failure in myxedema. Twenty patients with myxedema were admitted to the hospital with cardiac failure. The myxedema itself was the only recognizable cause of the failure in 4 patients. Of the remaining 16, generalized arteriosclerosis was noted in 12, with demonstrabl...