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Clustering of class I HLA molecules on the surfaces of activated and transformed human cells


, : Clustering of class I HLA molecules on the surfaces of activated and transformed human cells. Journal of Immunology 152(7): 3353-3360

We surveyed cells for the clusters of class I HLA molecules, HLA-1, which we have previously found on JY lymphoblasts. Two fluorescence techniques, fluorescence resonance energy transfer and electron exchange quenching, detected clustered HLA-1 molecules on activated normal B and T cells, on cells of B and T lymphoblast lines, and on transformed fibroblasts. No HLA-1 clusters were detectable in the surfaces of resting B or T cells or normal fibroblasts. HLA clustering correlates perfectly with the presence of the HC-10 epitope of beta-2-microglobulin (beta-2m)-free heavy chains at the cell surface although not with the amount of this epitope expressed. Clustering was reversed by exogenous beta-2m, but this did not change the amount of HC-10 bound. This suggests that a form of beta-2m-free heavy chain in equilibrium with both native HLA molecules and fully denatured HC-10-positive heavy chains is involved in HLA-1 cluster formation.

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PMID: 8144921


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