geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Co-existence of two types of chromosome in the bottom fermenting yeast, Saccharomyces pastorianus


, : Co-existence of two types of chromosome in the bottom fermenting yeast, Saccharomyces pastorianus. Yeast 14(10): 923-933

The bottom fermenting yeasts in our collection were classified as Saccharomyces pastorianus on the basis of their DNA relatedness. The genomic organization of bottom fermenting yeast was analysed by Southern hybridization using eleven genes on chromosome IV, six genes on chromosome II and five genes on chromosome XV of S. cerevisiae as probes. Gene probes constructed from S. cerevisiae chromosomes II and IV hybridized strongly to the 820-kb chromosome and the 1500-kb chromosome of the bottom fermenting yeast, respectively. Five gene probes constructed from segments of chromosome XV hybridized strongly to the 1050-kb and the 1000-kb chromosomes. These chromosomes are thought to be S. cerevisiae-type chromosomes. In addition, these probes also hybridized weakly to the 1100-kb, 1350-kb, 850-kb and 700-kb chromosome. Gene probes constructed from segments including the left arm to TRP1 of chromosome IV and the right arm of chromosome II hybridized to the 1100-kb chromosomes of S. pastorianus. Gene probes constructed using the right arm of chromosome IV and the left arm of chromosome II hybridized to the 1350-kb chromosome of S. pastorianus. These results suggested that the 1100-kb and 1350-kb chromosomes were generated by reciprocal translocation between chromosome II and IV in S. pastorianus. Three gene probes constructed using the right arm of chromosome XV hybridized weakly to the 850-kb chromosome, and two gene probes from the left arm hybridized weakly to the 700-kb chromosome. These results suggested that chromosome XV of S. cerevisiae was rearranged into the 850-kb and 700-kb chromosomes in S. pastorianus. These weak hybridization patterns were identical to those obtained with S. bayanus. Therefore, two types of chromosome co-exist independently in bottom fermenting yeast: one set which originated from S. bayanus and another set from S. cerevisiae. This result supports the hypothesis that S. pastorianus is a hybrid of S. cerevisiae and S. bayanus.

US$19.90

PMID: 9717238

DOI: 10.1002/(SICI)1097-0061(199807)14:10<923::AID-YEA298>3.0.CO;2-I


Other references

Castro, P.; Creighton, C.J.; Ozen, M.; Berel, D.; Mims, M.P.; Ittmann, M., 2009: Genomic profiling of prostate cancers from African American men. African American (AA) men have a higher incidence and significantly higher mortality rates from prostate cancer than white men, but the biological basis for these differences are poorly understood. Few studies have been carried out to determine wh...

Hajirasouliha, I.; Hormozdiari, F.; Alkan, C.; Kidd, J.M.; Birol, I.; Eichler, E.E.; Sahinalp, S.Cenk., 2010: Detection and characterization of novel sequence insertions using paired-end next-generation sequencing. In the past few years, human genome structural variation discovery has enjoyed increased attention from the genomics research community. Many studies were published to characterize short insertions, deletions, duplications and inversions, and asso...

Kriger, A.G.; Smirnov, A.V.; Akhtanin, E.A.; Varava, A.B., 2017: A true giant aneurysm of gastro-duodenal artery. Khirurgiia: 98-100

Green A.L., 1971: Inhibition of rat and mouse brain mono amine oxidases by plus amphetamine. Biochemical Journal 121(3): 37P-38P

Albaek, N.; Ravn, J.; Freitag, M.; Thomasen, H.; Christensen, N.K.; Petersen, M.; Nielsen, P., 2003: Bi- and tricyclic nucleoside derivatives restricted in S-type conformations and obtained by RCM-reactions. Ring-closing metathesis (RCM) is applied as a new and powerful technology in the construction of nucleoside analogues that are conformationally restricted in S-type conformations due to additional 3',4'- and/or 3',5'-linkages.

Shaywitz, S.; Shaywitz, B.; Wietecha, L.; Wigal, S.; McBurnett, K.; Williams, D.; Kronenberger, W.G.; Hooper, S.R., 2016: Effect of Atomoxetine Treatment on Reading and Phonological Skills in Children with Dyslexia or Attention-Deficit/Hyperactivity Disorder and Comorbid Dyslexia in a Randomized, Placebo-Controlled Trial. Evaluated the effects of atomoxetine on the reading abilities of children with dyslexia only or attention-deficit/hyperactivity disorder (ADHD) and comorbid dyslexia. Children aged 10-16 years (Nā€‰=ā€‰209) met Diagnostic and Statistical Manual of...

Guo, H-Wei.; Xu, J-Ping.; Song, Y-Hu.; Shen, X-Dong.; Li, S-Jun.; Hu, S-Shou., 2007: Repair of anomalous origin of left coronary artery from the pulmonary artery. Patients with anomalous origin of the left coronary artery from the pulmonary artery often have mitral valve regurgitation. Although establishing dual coronary circulation is the procedure of choice, there remains controversy as to how the mitral...

Ladd, M.R.; Pajewski, N.M.; Becher, R.D.; Swanson, J.M.; Gallaher, J.R.; Pranikoff, T.; Neff, L.P., 2013: Delays in treatment of pediatric appendicitis: a more accurate variable for measuring pediatric healthcare inequalities?. Racial and socioeconomic factors may cause barriers to healthcare access that result in delayed treatment. Because perforated appendicitis (PA) in children is thought to result from delays in treatment, it is often used as an index of barrier to a...

Lee, P-Hsuan.; Lin, T-Tung.; Chiou, W-Liang., 2008: Phenology of 16 species of ferns in a subtropical forest of northeastern Taiwan. A knowledge of fern phenology promotes understanding of the biology and ecology of ferns. In this study, the phenology of 16 fern species in a subtropical broadleaf forest (N24 degrees 46', E121 degrees 34') in northeastern Taiwan was mo...

Kruglikov G.G.; Pankova V.B.; Arutyunov V.D., 1977: Cytological and histological studies in diagnosis of allergic rhinopathies of chemical etiology. Vestnik Otorinolaringologii 5: 95-99