geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Coexistence of two biochemically distinct phospholipase A2 activities in human platelet, monocyte, and neutrophil


, : Coexistence of two biochemically distinct phospholipase A2 activities in human platelet, monocyte, and neutrophil. Biochemistry and Cell Biology 71(7-8): 331-339

The cell-associated phospholipase A-2 (PLA-2) activities of the human platelet, neutrophil, and monocyte were simultaneously characterized, utilizing the biochemical differences observed between the 14 kDa (kilodalton), type II PLA-2 isolated from inflammatory human synovial joint fluid (HSF) and the arachidonic acid (AA) specific, 85-kDa high molecular mass (HMM) PLA-2 isolated from the cytosol of a U937 monocytic cell line. The HSF PLA-2 can be distinguished from the HMM PLA-2 by its resistance to acid treatment, sensitivity to a sulfhydryl reducing agent, lack of preference for the fatty acid on the sn-2 position of phospholipid substrate, and inhibition by the C-7 phosphonate-phospholipid transition-state PLA-2 inhibitor. Evaluation of all three cell types revealed that HMM-like PLA-2 activity was found predominantly in the cytosolic fractions, although detection in neutrophil cytosol required more concentrated preparations and the use of high specific activity (3H)AA-labeled Escherichia coli. HSF-PLA-2-like activity was measured in microsomal and cytosolic fractions of all three cell types, but was found in neutrophil cytosol only after treatment with acid. Further HMM-PLA-2-specific interfering agents in neutrophil cytosol were observed and exemplifies one problem in assigning the existence of this enzyme in crude broken cell preparations using activity measurements alone. The role that these two enzymes play in eicosanoid production of the respective cell types remains to be studied.

US$29.90

PMID: 8123250


Other references

Mirza, N.; Machtay, M.; Devine, P.A.; Troxel, A.; Abboud, S.K.; Doty, R.L., 2007: Gustatory impairment in patients undergoing head and neck irradiation. To determine whether radiation alters taste function and structure. Prospective, longitudinal study. Testing prior to starting radiation, and 2 weeks, 2 months, and 6 months after completing radiation. Relative to controls, patients had lower tast...

Y.C.uangXing; Shi XiangGang, 2002: Two species: Camellia gigantocarpa Hu and Camellia crapnelliana Tutcher. In 1981, Professor Chang Hong-ta combined Camellia gigantocarpa with C. crapnelliana. The morphological differences between the 2 species based on several years of observation are discussed.

Morse, D.R.; Furst, M.L.; Koren, L.Z.; Bolanos, O.R.; Esposito, J.V.; Yesilsoy, C., 1987: Comparison of diflunisal and an aspirin-codeine combination in the management of patients having one-visit endodontic therapy. One hundred seventy-nine patients with asymptomatic or mildly symptomatic endodontic disease had single-visit therapy and were given either diflunisal (n = 94) or aspirin with codeine (n = 85) to control posttreatment pain. In this open-label, ran...

Whitehead, L.C., 1927: Notes on prairie-dogs. At Barstow, Texas, prairie dogs were observed to eat grasshoppers. Flooding of fields may not completely fill burrows.

Batdorf, David B., 2000: Scleral band and method for making. An elongate elastomeric band having unitary construction which construction includes a silicone sponge differentially compressive portion disposed along the length thereof. The band is dimensioned to encircle the eye of a patient having a detached...

Weitzman, S., 1983: Use of epidemiology in primary care. There is increasing interest in developing a stronger relationship between epidemiology and primary care. The practice of primary care, either individual-, family- or community-oriented, goes from examination and definition of health problems thro...

Ball, E.; Caivano, C.K., 2008: Internal medicine: guidance to the diagnosis and management of restless legs syndrome. Restless legs syndrome (RLS) is a chronic neurologic disorder, with a prevalence rate in the general population of 5% to 10%. The diagnosis of RLS is straightforward; it is based on symptom history alone and uses the four essential diagnostic cri...

Inomata, N., 1991: Hybrid progenies of the cross, Brassica campestris X B. oleracea. IV. Crossability of F2, B1 and hybrid plants, and their progenies. Crossability was examined in the F2, BC1 and hybrid plants, and the somatic chromosomes of their progenies were investigated. The F2 and BC1 plants were obtained in the self- and open pollinations, and in the F1 hybrids backcrossed to both parents...

Roger, L.M.ubois, J.; Thapon, J.; Brule, G., 1978: Hydrolysis of lactose contained in the milk or whey ultrafiltrate in a membrane enzymatic reactor. Annales de la nutrition et de l'alimentation2(2-3): 657-669

Dresel P.E.an; Murray Chris, 1998: Groundwater monitoring network design using stochastic simulation. Approximately 100 square miles of groundwater at the U. S. Department of Energy's Hanford Site, WA have been contaminated with tritium, iodine-129, and nitrate originating from waste disposal at the 200-East Area. Groundwater monitoring has b...