geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Construction of temperature and Zn-dependent human stromal cell lines that amplify hematopoietic precursors from cord blood CD34+ cells


, : Construction of temperature and Zn-dependent human stromal cell lines that amplify hematopoietic precursors from cord blood CD34+ cells. Experimental Hematology 26(6): 534-540

Forty-five human stromal cell lines were established from long-term bone marrow cultures transformed with a new vector, pNu MTSVts, which contains the Zn-inducible metallothionein promoter and the temperature-dependent SV40 T antigen from SV40 A58 mutant. Six of these cell lines were studied because of their growth capacity. All cell lines differed with respect to growth potential, expression of cell surface markers, and cytokine transcripts. Major histocompatibility complex (MHC) class I, CD29, CD49d, and CD51 were present on all stromal cell lines, MHC class II and CD34 were consistently absent, and CD11a (LFA-1), CD18 (ICAM-1R), CD54 (ICAM-1), CD58 (LFA-3) CD56 (N-CAM), CD106 (V-CAM), laminin, and collagen IV were diversely expressed. All cell lines contained interleukin (IL)-1alpha, IL-1beta, IL-2, IL-5, and macrophage colony-stimulating factor (M-CSF) transcripts, whereas granulocyte M-CSF, TNFalphat, IL-3, IL-4, and IL-7 were diversely expressed. The most characteristic feature of these cells was their varying capacity to expand cord blood CD34+ cells. One of these stromal cell lines ensured more than twofold expansion of the initial CD34+CD10-CD19- population in the first 2 weeks. Differentiation toward the B cell lineage was limited, producing only very small numbers of CD19+ cells after 6 weeks of culture.

US$29.90

PMID: 9620287


Other references

Anonymous, 1987: Easy As ABC. The Resuscitation Council of the UK has decided that the life-saving procedure can now be conducted alphabetically. ABC stands for airway, breathing and circulation, which should be tackled in that order. But at least one council member is suspici...

Luger, T.A., 1985: Diagnosis and therapy of syphilis as well as other venereal diseases. Gynakologische Rundschau 25(1): 58-65

Madan, A.K.; Ternovits, C.A.; Speck, K.E.; Pritchard, F.Elizabeth.; Tichansky, D.S., 2006: Laparoscopic lumbar hernia repair. Lumbar hernias are rare clinical entities that often pose a challenge for repair. Because of the surrounding anatomy, adequate surgical herniorraphy is often difficult. Minimally invasive surgery has become an option for these hernias. Herein, we...

Erickson, G.E.; Klopfenstein, T.J.; Milton, C.T.; Hanson, D.; Calkins, C., 1999: Effect of dietary phosphorus on finishing steer performance, bone status, and carcass maturity. Yearling crossbred steers (n = 60; 386 kg) were individually fed in a completely randomized experimental design to determine their P requirement. Treatments were in a factorial arrangement with two levels of Ca (.35 or .70% of DM) and five concent...

Anonymous, 1998: Proceedings of the Conference on Early Childhood Caries. Bethesda, Maryland, USA. October 1997. Community Dentistry and Oral Epidemiology 26(1 Suppl): 1-119

Dobbs, A.J.; Grant, C., 1976: Report on the burning of wood treated with wood preservatives containing copper, chromium and arsenic. In order to determine the potential hazards involved in airborne emissions and ash residue, small-scale experimental burning of CCA-treated Baltic redwood [Pinus sylvestris], in the form of sapwood particles >2 mm in size, was done at the Princ...

Sharma, D.; Southworth, D.R.; Green, R., 2003: EF-G-independent reactivity of a pre-translocation-state ribosome complex with the aminoacyl tRNA substrate puromycin supports an intermediate (hybrid) state of tRNA binding. Following peptide-bond formation, the mRNA:tRNA complex must be translocated within the ribosomal cavity before the next aminoacyl tRNA can be accommodated in the A site. Previous studies suggested that following peptide-bond formation and prior t...

Ugarte, J., 1988: Sugarcane and residues from its harvesting as cattle feed. The ME of sugarcane per unit of DM is similar to that of forage grasses, and its DM digestibility in vitro may exceed 70%, but supplementation with N (as urea) is imperative for feeding cane to cattle. Weight gain of steers and milk yield from dai...

Sadowski, B., 1976: Central control of hunger and satiation and energy metabolism. Acta Physiologica Polonica 27(6): 129-145

Maillieux Eugene, 1938: Compte rendu de lexcursion de la Societe belge de Geologie a Sart-Bernard et a Naninne, le 30 juin 1938. Field notes on the exposures of the Sambre-et-Meuse Silurian band in the Sart-Bernard and Naninne areas, Belgium.