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Cytosolic free Ca2+ oscillations induced by diadenosine 5',5"'-P1,P3-triphosphate and diadenosine 5',5"'-P1,P4-tetraphosphate in single rat hepatocytes are indistinguishable from those induced by ADP and ATP respectively

, : Cytosolic free Ca2+ oscillations induced by diadenosine 5',5"'-P1,P3-triphosphate and diadenosine 5',5"'-P1,P4-tetraphosphate in single rat hepatocytes are indistinguishable from those induced by ADP and ATP respectively. Biochemical Journal 310: 629-635

Diadenosine 5',5"'-P-1,P-3-triphosphate (Ap-3A) and diadenosine 5',5"'-P-1,P-4-tetraphosphate (Ap-4A) induce distinctive patterns of (Ca-2+)-i oscillations in single rat hepatocytes. We show here that (Ca-2+)-i oscillations induced by Ap-3A and ADP are indistinguishable and that (Ca-2+)-i oscillations induced by Ap-4A closely resemble those induced by ATP. These similarities embrace the following: (1) ADP and Ap-3A invariably induce (Ca-2+)-i transients of short duration (approx. 9 s). Ap-4A, like ATP, can induce, depending upon the individual cell, either transients of short duration (approx. 9 s), transients of much longer duration or a mixture of short and long transients within a single response. We show here that the pattern of oscillations induced by Ap-4A is similar to that induced by ATP in the same hepatocyte. (2) Elevated intracellular cyclic AMP concentration modulates Ap-3A-induced transients, like ADP-induced transients, through an increase in both the peak (Ca-2+)-i and the frequency of the transients. In contrast, Ap-4A-induced transients, like ATP-induced transients, develop an increased duration or a sustained rise in (Ca-2+)-i, with no rise in peak (Ca-2+)-i. (3) Ap-3A-induced transients, like ADP-induced transients, are abolished by low concentrations of the phorbol ester 4-beta-phorbol 12,13-dibutyrate (PDB; 5-10 nM), whereas long Ap-4A-induced transients, like long ATP-induced transients, are refractory to high concentrations of PDB (100 nM). We propose that the (Ca-2+)-i oscillations induced in rat hepatocytes by Ap-3A are mediated by the same purinoceptor that mediates the effects of ADP, whereas the oscillations induced by Ap-4A are mediated by the same purinoceptor(s) that mediate the effects of ATP.


PMID: 7654204

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