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Detection of AB(O) antigens and keratin polymorphism in forensic medical examination of hairs


, : Detection of AB(O) antigens and keratin polymorphism in forensic medical examination of hairs. Sudebno Meditsinskaya Ekspertiza 40(1): 31-33

Hair proteins were extracted by buffer consisting of 8 M urea, 0.025 M dithiothreitol, 2% sodium dodecylsulfate, and 0.01 M tris-HCl pH 10.4. The proteins were analyzed by gradient polyacrylamide gel electrophoresis. A single 5 cm hair, previously subjected to AB0 blood group analysis by the absorption-elution test, was sufficient for assessing the keratin phenotype.

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