geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Detection of ascorbate peroxidase activity in native gels by inhibition of the ascorbate-dependent reduction of nitroblue tetrazolium


, : Detection of ascorbate peroxidase activity in native gels by inhibition of the ascorbate-dependent reduction of nitroblue tetrazolium. Analytical Biochemistry 212(2): 540-546

A method for the detection of ascorbate peroxidase activity in native electrophoretic gels is described. The assay is based on the ability of ascorbate peroxidase to prevent the ascorbate-dependent reduction of nitroblue tetrazolium in the presence of H-2O-2. The method was found to be both sensitive (detection of less than 0.01 units of ascorbate peroxidase activity) and specific for ascorbate peroxidase activity. The application of the method for the detection of ascorbate peroxidase activity in protein extracts from several plant sources was investigated by comparing staining for activities of ascorbate peroxidase, horseradish peroxidase, and ascorbate oxidase and by immunodetection of ascorbate peroxidase in these extracts.

US$19.90

PMID: 8214598

DOI: 10.1006/abio.1993.1366


Other references

Reppas, J.B.; Tootell, R.B.H.; Dale, A.M.; Rosen, B.R., 1995: Cortical contributions to the interpretation of visual image motion and the execution of pursuit eye movements. Investigative Ophthalmology & Visual Science 36(4): S205

Minnaganti, V.R.; Patel, P.J.; Iancu, D.; Schoch, P.E.; Cunha, B.A., 2000: Necrotizing fasciitis caused by Aeromonas hydrophila. Aeromonas Hydrophila is a gram-negative bacillus commonly found in soil, sewage, and fresh or brackish water in many parts of the United States. In healthy people, the most common clinical manifestations attributed to Aeromonas are diarrhea and so...

Oriel, J.D.; Ridgway, G.L.; Goldmeier, D.; Felmingham, D., 1982: Treatment of gonococcal urethritis in men with a rifampicin-erythromycin combination. A group of men with uncomplicated gonococcal urethritis were treated with a single oral dose of rifampicin (900 mg) and erythromycin stearate (1 g). Neisseria gonorrhoeae was isolated from four of 108 men at the first follow-up examination, and fr...

Culmsee, C.; Monnig, J.; Kemp, B.E.; Mattson, M.P., 2001: AMP-activated protein kinase is highly expressed in neurons in the developing rat brain and promotes neuronal survival following glucose deprivation. Adenosine monophosphate-activated protein kinase (AMPK) is a member of metabolite-sensing kinase family that plays important roles in responses of muscle cells to metabolic stress. AMPK is a heterotrimer of a catalytic alpha subunit (alpha1 or alp...

Robinson Peter; Elbert David C., 1992: The Bernardston Nappe and Brennan Hill Thrust in the Connecticut Valley and root zones on the east limb of the Bronson Hill Anticlinorium. Contribution - Geology Department, University of Massachusetts 66, Vol

Johns Patricia J.; Woodfork Larry D.; Patchen Douglas G.; Avary Katharine Lee; Aston Kenneth A., 2001: Sistersville, West Virginia; 1893 oil capitol of the world. Oil-Industry History 2(1): 91

Rosenthal, Sol Roy, 1942: On a method of decreasing the reactivity to tuberculin by the inhalation of fumes from a boiling suspension of tubercle bacilli. Federation Proc Pt Ii 1(1): 182-183

Madsen J.S.; Reimann I.; Svalastoga E., 1991: Delayed ossification of the femoral head in dogs with hip dysplasia. Journal Of Small Animal Practice. 32(7): 351-354

Hedtler, W.; Berchtold, C.; Wey, W., 1988: Significance of computerized tomography and sonography in the staging (TNM classification) of cancers of the tongue and floor of the mouth. Computerized tomography and sonography were used for pretherapeutic staging of 40 patients with cancer of the tongue. The size of the tumors and invasion of cervical lymph nodes were assessed, and both methods were compared in relation to the stag...

Woodland, H.R., 1989: Mesoderm formation in Xenopus. Cell 59(5): 767-770