geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Distribution of the serotonin 5-HT-2 receptor family mRNAs: Comparison between 5-HT-2A and 5-HT-2C receptors


, : Distribution of the serotonin 5-HT-2 receptor family mRNAs: Comparison between 5-HT-2A and 5-HT-2C receptors. Molecular Brain Research 23(1-2): 163-178

Because of their similarities, serotonin 5-HT-2, 5-HT-1C, and the recently described 5-HT-2F receptors have been classified as members of the 5-HT-2 receptor family, and they have been renamed 5-HT-2A, 5-HT-2C and 5-HT-2B, respectively. The regional distribution and cellular localization of mRNA coding for the members of 5-HT-2 receptor family were investigated in consecutive tissue sections from the rat brain by in situ hybridization histochemistry. No evidence for the expression of 5-HT-2B receptor was found. High levels of 5-HT-2A (formerly 5-HT-2) receptor mRNA were observed only in few areas, as the frontal cortex, piriform cortex, ventro-caudal part of CA3, medial mammillary nucleus, the pontine nuclei and the motor cranial nerve nuclei in the brainstem, and the ventral horn of the spinal cord. The distribution of 5-HT-2A receptor mRNA is generally in good agreement with that of the corresponding binding sites, although discrepancies were sometimes observed. 5-HT-2C (formerly 5-HT-1C) mRNA was present at very high levels in the choroid plexuses. However, very high levels were also seen in many other brain regions, as the retrosplenial, piriform and entorhinal cortex, anterior olfactory nucleus, lateral septal nucleus, subthalamic nucleus, amygdala, subiculum and ventral part of CA3, lateral habenula, substantia nigra pars compacta, several brainstem nuclei and the whole grey matter of the spinal cord. These results confirm and extend previous observations that 5-HTc receptor mRNA is present in many brain areas in addition to those autoradiographically shown to have the corresponding binding sites and that 5-HT-2C receptor subtype is a principal 5-HT receptor in the brain. From the comparison between their distributions, 5-HT-2A and 5-HT-2C receptor mRNAs appeared to be expressed in distinct but overlapping sets of brain regions. Both mRNAs coexisted at high levels in the anterior olfactory nucleus, piriform cortex, endopiriform nucleus, claustrum, pyramidal cell layer of the ventral part of CA3, taenia tecta, substantia nigra pars compacta, and several brainstem nuclei. In other regions both mRNAs were present but with different distributions, as the caudate-putamen. These results are also discussed in relation to the physiological meaning of the existence of two so similar receptor subtypes in the brain.

US$19.90

PMID: 8028479

DOI: 10.1016/0169-328X(94)90223-2


Other references

Grenne Tor, 1977: Geochemistry of metavolcanic rocks in the Fundsjo Group-Hersjo Formation and the Storen Group of eastern and western Trondheim. Pages 62-63 1977

Fremstad, E.; Elven, R., 2004: Perennial lupins in Fennoscandia. Three North American perennial species of Lupinus occur as established aliens in Fennoscandia: Lupinus nootkatensis, L. perennis and L. polyphyllus. Lupinus nootkatensis and L. perennis were introduced mainly in connection with railroad constructi...

Avila Rodriguez A.; Reiriz Paz J.M.; Jimenez Gutierrez J.; Martinez Cantarero J.; Garcia Martin R., 1987: Sonographic features of sclerosing cholangitis. Radiologia (Madrid) 29(5): 299-302

Narahashi, T.; Anderson, N.C.; Moore, J.W., 1966: Tetrodotoxin does not block excitation from inside the nerve membrane. Tetrodotoxin does not block the action potential or membrane sodium current when internally perfused through the giant axon of a squid at much higher concentrations than those required for blocking by external application. It is suggested that the...

Moens, P.B.; Heyting, C.; Dietrich, A.J.; van Raamsdonk, W.; Chen, Q., 1987: Synaptonemal complex antigen location and conservation. The axial cores of chromosomes in the meiotic prophase nuclei of most sexually reproducing organisms play a pivotal role in the arrangement of chromatin, in the synapsis of homologous chromosomes, in the process of genetic recombination, and in th...

Zhou, Q.; Ye, X.; Sun, R.; Matsumoto, Y.; Moriyama, M.; Asano, Y.; Ajioka, Y.; Saijo, Y., 2014: Differentiation of mouse induced pluripotent stem cells into alveolar epithelial cells in vitro for use in vivo. Alveolar epithelial cells (AECs) differentiated from induced pluripotent stem cells (iPSCs) represent new opportunities in lung tissue engineering and cell therapy. In this study, we modified a two-step protocol for embryonic stem cells that resul...

Zhao, D.; Tian, Y.; Li, P.; Wang, L.; Xiao, A.; Zhang, M.; Shi, T., 2016: MicroRNA-203 inhibits the malignant progression of neuroblastoma by targeting Sam68. Neuroblastoma (NB) is the most common solid extracranial tumor in children. However, the molecular mechanism of NB remains to be elucidated. In the present study, reverse transcription quantitative polymerase chain reaction data demonstrated that...

Yang, P.; Chen, Z.; Shan, Z.; Ding, X.; Liu, L.; Guo, J., 2014: Effects of FMN riboswitch on antioxidant activity in Deinococcus radiodurans under H₂O₂ stress. The flavin mononucleotide (FMN) riboswitch is structured noncoding RNA domains that control gene expression by selectively binding FMN or sensing surrounding changes without protein factors, which are involved in the biosynthesis and transport of...

Kolesova V.I.; Petrova A.A.; Efendieva M.A. (Efendiyeva M.A., 1979: Investigations of space structure of the geomagnetic field in the North Pacific. Abstracts of Papers - Pacific Science Congress 14: 9

Durairaj, V.; Radhabai, K.; Alagappan, R.; Koteeswaran, A.; Venkataraman, V.; Kannan, K., 1984: Adrenal cortical function and reserve in lepromatous leprosy. 22 chronic Lepromatous Leprosy patients of over 10 years duration, 17 non-reactional and 5 in reactional state who have not taken steroids as part of treatment were selected for the study. Serum cortisol was estimated by Radio-immuno-assay. Sample...