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Effect of interferon-alpha2b on guinea pig wound closure and the expression of cytoskeletal proteins vivo

, : Effect of interferon-alpha2b on guinea pig wound closure and the expression of cytoskeletal proteins vivo. Wound Repair & Regeneration 6(3): 202-212, May-June

Scar contraction following the healing of deep partial-thickness or full-thickness dermal injury is a leading cause of functional and cosmetic morbidity. The therapeutic use of interferon for the treatment of fibroproliferative disorders associated with scar contraction, including hypertrophic scar, has been suggested because of its antifibrotic properties. Treatment of fibroblasts with interferon has been shown to reduce f he rate and extent of contraction using the in vitro fibroblast-populated collagen lattice model. In order to establish the effect of interferon-alpha2b on full-thickness wound contraction in vivo, osmotic pumps loaded with interferon or sterile saline were implanted intraperitoneally in guinea pigs. Seven days following implantation, six full-thickness punch biopsy wounds were created and were monitored by daily assessment of the wound. There was a significant reduction in the rate of wound contraction in the interferon-treated animals after day 3 (p < 0.01). Western blot analysis was used to quantitate selected cytoskeletal proteins in the normal skin and tissue biopsied from the wound at days 7, 14, and 21 postinjury. The amount of vimentin in the contracted wound increased following injury as compared with the amount present in normal skin (p < 0.0001); however, the relative amounts of the myofibroblast-associated cytoskeletal proteins alpha-smooth muscle actin and smooth muscle myosin were less than those found in normal, uninjured skin. By using vimentin to adjust the levels of cytoskeletal proteins for the increase in cellularity in the wounds, both alpha-smooth muscle actin and smooth muscle myosin significantly increased after closure of the wounds on day 14, as compared with the open-wound stage (day 7), before further reductions occurred with remodeling on day 21. Measurement of apoptotic cells using the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay revealed an increase in apoptosis in the interferon-alpha2btreated animals at 21 days following wounding (p < 0.001), which did not colocalize with alpha-smooth muscle actin staining. Taken together, these findings suggest that interferon-alpha2b inhibits wound contraction in vivo, not through an appreciable alteration in myofibroblast number or cytoskeletal protein expression, but possibly through a reduction in fibroblast cellularity by the induction of apoptosis.


PMID: 9776864

Other references

Aragon P.E.E.; Baudoin C., 1989: Some reproductive aspects of two squirrel species of the genus spermophilus rodentia sciuridae in a sympatric zone of the chihuahua desert. The reproductive cycle, litter size, recruitment, juvenile growth and reproductive behavior of Spermophilus spilosoma and S. mexicanus were studied at the Mapimi Biosphere Reserve during 1986-1988. 267 S. spilosoma and 132 S. mexicanus were captur...

Bijlsma, Rob., 1994: Precarious foothold of Montagu's harrier in the Netherlands, and elsewhere in Europe. Talon Talk. September; 3: 1p.

Anonymous, 2012: Evaluation of the commonly used acaricides against different stages of the cattle tick Boophilus microplus by using different in vitro tests. The evaluation of the efficacy of three commonly used acaricides viz. cypermethrin, deltamethrin (0.05, 0.1 and 0.2% concentrations) and amitraz (0.1, 0.2 and 0.3% concentrations) was conducted against the cattle tick Boophilus microplus. Three in...

Kartavova N.S., 1986: Directed receptor status modification in experimental mammary tumors during their therapy. Uicc (Union Internationale Contre Le Cancer, International Union Against Cancer) 14th International Cancer Congress, Budapest, Hungary, Aug 21-27, Abstracts, Lectures, Symposia And Free Communications, Vols 1, 2, 3, Late Abstracts, And Register Xvi+479p (Vol 1); Xvi+298p (Vol 2); Xvi+531p (Vol 3); 15p (Late Abstracts); 40p (Register) S Karger Ag: Basel, Switzerland; New York, N Y , Usa; Akademiai Kiado: Budapest, Hungary Paper 1203

Ustaoglu, S.; Rennert, B., 2002: The apparent nutrient digestibility of diets containing fish meal or isolated soy protein in sterlet (Acipenser ruthenus). Feeding experiments were conducted to determine the apparent digestibility coefficients (ADCs) for sterlet (Acipenser ruthenus) fed diets containing different protein sources (fish meal and isolated soy protein). The ADCs were measured using the i...

Zaffran, J., 1966: A new bellflower of the Cretan flora Campanula aizoides. A diagnosis of the new species is given, with a map showing the region of its discovery, sketches, and photographs.

Gregory, L.J.; Yaguez, L.; Altmann, C.; Williams, S.C.R.; Ng, V.W.K.; Brammer, M.J.; Thompson, D.G.; Aziz, Q., 2001: Identifying the neural network for selective attention to visceral sensation using functional magnetic resonance imaging. Gastroenterology 120(5 Supplement 1): A 23, April

Krueger, P.D.; Kim, T.S.; Sung, S-Sang.J.; Braciale, T.J.; Hahn, Y.S., 2015: Liver-resident CD103+ dendritic cells prime antiviral CD8+ T cells in situ. The liver maintains a tolerogenic environment to avoid unwarranted activation of its resident immune cells upon continuous exposure to food and bacterially derived Ags. However, in response to hepatotropic viral infection, the liver's ability...

Theppawong, A.; D.V.eese, R.; Vannecke, L.; Grootaert, C.; Van Camp, J.; D'hooghe, M., 2016: Synthesis and biological assessment of novel N-(hydroxy/methoxy)alkyl β-enaminone curcuminoids. NlmCategory="UNASSIGNED">Curcumin, a natural compound extracted from the rhizomes of Curcuma Longa, is known to display pronounced anticancer activity but lacks good pharmacokinetic properties. In that respect, augmenting the water so...

Kolodiy V.V.; Sivan T.P., 1973: Pore solutions in Tertiary sediments of the Crimean Plain. Chemical composition of pore and connate waters, USSR.