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Effect of phorbol 12-myristate 13-acetate (PMA) on the phosphoinositol (PI) system in Tetrahymena. Study of the 32P incorporation and breakdown of phospholipids


, : Effect of phorbol 12-myristate 13-acetate (PMA) on the phosphoinositol (PI) system in Tetrahymena. Study of the 32P incorporation and breakdown of phospholipids. Cell Biochemistry and Function 13(2): 85-89

Phorbol 12-myristate 13-acetate (PMA) treatment elicited an increased 32P incorporation into phospholipids namely phosphatidyl-inositol (PI); phosphatidyl-inositol-4-phosphate (PIP); phosphatidyl-inositol-4,5-bis-phosphate (PIP-2); phosphatidyl-acid (PA); phosphatidyl-choline (PC) and phosphatidyl-ethanolamine (PE) particularly at the 20-30th min after treatment. The ratio of members of the phosphoinositol system, especially PIP and PI, related to the total phospholipid content was increased. PMA (2 times 10-7 M) was the most effective of the three concentrations tested. The results call attention to the presence of a working phosphoinositol system in Protozoa.

US$19.90

PMID: 7758152

DOI: 10.1002/cbf.290130204


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