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Effect of photodynamic therapy on the endothelium-dependent relaxation of isolated rat aortas


, : Effect of photodynamic therapy on the endothelium-dependent relaxation of isolated rat aortas. Cancer Research 53(11): 2548-2552

The early vascular effects of photodynamic therapy (PDT) include transient vasoconstriction and platelet aggregation. Since endothelium-derived relaxing factor (EDRF) is a potent vasodilator and inhibitor of platelet aggregation, we questioned whether PDT impairs the production of EDRF. To study this possible effect of PDT, endothelium-dependent relaxations of thoracic aortas obtained from male Wistar rats were determined. The aortic rings were connected to a isometric force transducer, exposed to various doses of Photofrin porfimer sodium (Photofrin) (0.1-1.0 microgram/ml), and illuminated with red light (wavelength > 610 nm, 14.6 +/- 1.5 mW/cm2) for different time periods (5-25 min). Endothelium-dependent relaxation was induced by acetylcholine in precontracted aortic rings. This EDRF-mediated relaxation was decreased after PDT in a light dose- and drug dose-dependent manner. Light microscopic examination did not show loss of endothelial cells. Similar results were obtained with rat aortas exposed to Photofrin in vivo and illuminated in vitro. Direct smooth muscle relaxation induced with sodium nitroprusside was not impaired, showing that PDT did not reduce the ability of smooth muscles to relax. No effect on the contractile responses was found either. We conclude that PDT impairs the production or release of EDRF by the endothelium. This could play an important role in the initial events occurring in vivo during and after PDT.

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PMID: 8495418


Other references

Murphy, P., 1987: The Cincinnati Academy of Physical Education: Using Fitness to Aid Learning. A Cincinnati public school mixes a healthy dose of activity with a rigorous academic curriculum. The result, school officials hope, will be adults who are both physically and mentally fit.

Semenov, P.V.; Gomoyunova, N.P.; Tarasenko, N.N., 1975: Flight radius of Oestrus ovis. After releasing specimens of Oestrus ovis marked with a red spot, the authors recovered one that had travelled 3 km and another that had travelled (in four days) 30 km. The experiments were carried out in June in the Uimonsk steppe of Siberia.

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Nienartowicz, A.; Sych, Z., 1979: Evaluation of the palpation method of estimation of fetal weight according to the Johnson's method in authors' own modification. Ginekologia Polska 50(6): 487-494

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