geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Effects of adrenomedullin on the human adrenal glands: an in vitro study


, : Effects of adrenomedullin on the human adrenal glands: an in vitro study. Journal of Clinical Endocrinology and Metabolism 82(4): 1167-1170

Numerous lines of evidence indicate that adrenal medulla exerts a paracrine control on the secretory activity of the cortex by releasing catecholamines and several regulatory peptides. Adrenomedullin (ADM) is contained in adrenal medulla of several mammalian species, including humans. Thus, we investigated whether human ADM1-52 exerts a modulatory action on steroid secretion of human adrenal cortex in vitro. Dispersed adrenocortical cells (obtained from the gland tail deprived of chromaffin cells) and adrenal slices (including both capsule and medulla) were employed. ADM specifically inhibited angiotensin II-stimulated aldosterone secretion of dispersed cells and enhanced basal aldosterone production by adrenal slices, minimal effective concentrations being 10(-7) and 10(-9) mol/L, respectively. These effects of ADM were suppressed by the CGRP1 receptor antagonist CGRP8-37 (10(-5) mol/L). Neither basal and ACTH-stimulated aldosterone secretion of dispersed cells nor agonist-enhanced aldosterone production by adrenal slices were affected by ADM, which also did not alter cortisol secretion of both types of adrenal preparations. ADM (10(-6) mol/L) blunted the aldosterone secretagogue action of the Ca2+ ionophore A23187 (10(-5) mol/L) on dispersed cells and adrenal slices. The beta-adrenoceptor antagonist l-alprenolol (10(-6) mol/L) suppressed aldosterone response of adrenal slices to 10(-7) mol/L isoprenaline and ADM. ADM concentration dependently raised epinephrine and norepinephrine release by adrenal slices, minimal effective concentration being 10(-9) mol/L. Collectively, these findings suggest that ADM, acting via the CGRP1 receptor subtype, exerts a direct inhibitory effect on angiotensin II-stimulated aldosterone secretion, which, when the integrity of adrenal tissue is preserved, is overcome and reversed by an indirect stimulatory action, conceivably involving the release of catecholamines by adrenal chromaffin cells.

US$19.90

PMID: 9100590

DOI: 10.1210/jcem.82.4.3854


Other references

Wiener S.L., 1974: Teaching of physical diagnosis. Annals of Internal Medicine 80(6): 772

Bashniak, V.V., 1990: Syntopy of the pancreas. Resulting from the examination of 309 patients, the author has established that the pancreas could displace to the region of the lesser sac with ensuing impairement of the blood outflow through the mesenteric veins. This causes the development of...

Kolomois'ka, M.B.; Latypova, G.A., 1993: Acute gastric dilatation in diabetes mellitus patients. Observations of fatal outcomes of acute gastric dilatation (AGD) in diabetes mellitus patients were described in connection with the rarity of the condition and difficulties in its diagnosis. It was found that not only gastrointestinal diseases (p...

Pothitirat,W.; Gritsanapan,W., 2008: Variability of curcuminoids antioxidative components in ethanolic turmeric extract determined by UV and HPLC methods. In Thailand, turmeric (<i>Curcuma longa</i>) is mainly used in the forms of capsules/tablets of the powder for herbal medicine while its extract is also used in herbal cosmetics and functional food. Thus the quality assessment of this...

Nye, E.R., 1986: Some hazards of heavy exercise: jogging and running--is it all good?. New Zealand Medical Journal 99(813): 847-847

Fernandez, O.; Murillo, M.L.; Sanchez, O.C., 2001: Intestinal absorption and biliary secretion of 5MTHF: effect of ethanol. The aim of this work was to study intestinal absorption, using the whole intestine in vivo with 5-methyltetrahydrofolate (5MTHF), and the subsequent appearance of this compound in the bile, in control and in ethanol-fed rats. The ethanol-fed rats...

Passerin d'Entreves, P., 1983: Faunae Ligusticae Fragmenta e Insectorum Liguriae species novae di Massimiliano Spinola note bibliografiche. Museo Regionale di Scienze Naturali Bollettino (Turin): 12: 215-226

Lu, M.; Quan, Z.; Liu, B.; Jiang, D.; Ou, Y.; Zhao, J., 2008: Establishment and assessment of the mouse model for spinal cord injury. To make a mouse model of traumatic spinal cord injury (SCI) by Allen's weight dropping (WD), which might be helpful for further research on the mechanism of SCI. A total of 180 healthy female mice, weighing 17 - 23 g (20 g on average), were r...

Mallet, J.F.; Rigault, P.; Padovani, J.P., 1984: Septic complications of osteoarticular surgery in children. Revue de Chirurgie Orthopedique et Reparatrice de L'appareil Moteur 70 Suppl 2: 101-104

Badran, A.M.; Jones, D.E., 1965: Polyethylene glycols-tannins interaction in extracting enzymes. Nature 206(984): 622-624