+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Effects of macrophage colony-stimulating factor on folliculogenesis in gonadotrophin-primed immature rats

, : Effects of macrophage colony-stimulating factor on folliculogenesis in gonadotrophin-primed immature rats. Journal Of Reproduction & Fertility. 104(2): 325-330

The effect of macrophage colony-stimulating factor (M-CSF) on folliculogenesis and ovulation was studied. Folliculogenesis and ovulation were induced in immature female rats with a s.c. injection of equine chorionic gonadotrophin (eCG), followed 48 h later by human chorionic gonadotrophin (hCG). The ovulation rate was measured after the following treatments. (1) Graded doses of human M-CSF (1-300 times 10-3 iu per rat) were administered i.p. daily for 3 consecutive days. (2) M-CSF (100 times 10-3 iu) was administered i.p. at designated times between 96 h before and 10 h after hCG injection. (3) Rabbit anti-human M-CSF polyclonal antibody (5 mu-g) was administered into the left ovarian bursa at designated times between 49 h before and 10 h after hCG injection. In addition, the effect of M-CSF on ovarian macrophages was investigated using immunohistochemistry with mouse anti-rat macrophage monoclonal antibody, TRPM-3. The treatment with M-CSF ( gt 30 times 10-3 iu per rat) significantly increased the ovulation compared with controls in a dose-dependent manner. This stimulatory effect of M-CSF was observed when it was administered between 96 h and 49 h before hCG injection. The ovarian intrabursal administration of anti-M-CSF antibody significantly inhibited the number of ovulated ova from the treated ovaries compared with either those from control rats or from the contralateral untreated ovaries between 24 h before and 3 h after hCG injection. The immunohistochemistry revealed that M-CSF increased the number of ovarian macrophages in growing follicles. The results suggest that M-CSF is involved in the process of folliculogenesis and that it promotes ovulation by influencing ovarian macrophages.


PMID: 7473425

DOI: 10.1530/jrf.0.1040325

Other references

Kobayashi, Y.; Naganuma, R.; Seki, S.; Aketa, K.; Ichimiya, T.; Namiki, A., 1998: Reduction of pain on injection of propofol: a comparison of fentanyl with lidocaine. We compared the effect of fentanyl and lidocaine on pain during injection of propofol. One hundred and sixty patients premedicated with midazolam were randomly allocated to one of four groups (n = 40, respectively); Group C, propofol 2; Gr...

Sepsenwol S.; Braun T., 1978: Sub cellular localization of adenylate cyclase activity in bovine sperm tails. George, William J And Louis J Ignarro (Ed ) Advances in Cyclic Nucleotide Research, Vol 9 Third International Conference on Cyclic Nucleotides New Orleans, La , Usa, July 17-22, 1977 Xxxii+799p Illus Raven Press: New York, N Y , Usa Isbn 0-89004-240-3 739

Zohar, D., 2010: Thirty years of safety climate research: reflections and future directions. Looking back over 30 years of my own and other safety-climate scholars' research, my primary reflection is that we have achieved an enormous task of validating safety climate as a robust leading indicator or predictor of safety outcomes acros...

Render, D., 2002: Community health. Keeping your community fit. Trustee 53(4): 27-28

Mabbett, T., 2000: Rodents in the feed mill? Deny access, use safe chemical control. The problems and possible solutions associated with rodent infestations in feed mills are outlined.

Zavázal, V., 1969: The changes in histaminopexy in the course of hyposensitization therapy. Zeitschrift für Immunitatsforschung, Allergie und Klinische Immunologie 137(4): 365-370

Vasquez, O.N.lson, Jr; Hamilton,. Jr.;, 2002: Regression analysis to determine the effects of land characteristics on farmland values in south-central Idaho. Journal of the American Society of Farm Managers and Rural Appraisers: 9-77

Passey, R.B., 1994: CLIA--Part 3. Is CLIA understood by clinical laboratorians?. Mlo: Medical Laboratory Observer 26(7): 54-59

Kondratiuk, T.P.; Kurskiĭ, M.D.; Litvinenko, L.T., 1974: Properties of rabbit muscle aldolase in vitamin-E-deficient dystrophy. Ukrains'kyi Biokhimichnyi Zhurnal 46(4): 411-417

Devlin, J., J.; Liang, A.; Trinh, L.; Polokoff, M., A.; Senator, D.; Zheng, W.; Kondracki, J.; Kretschmer, P., J.; Morser, J.; Lipson, S., E.; Spann, R.; Loughlin, J., A.; Dunn, K., V.; Morrissey, M., M., 1996: High capacity screening of pooled compounds: Identification of the active compound without re-assay of pool members. A matrix-based compound pooling and deconvolution method has been developed that significantly increased the efficiency of a high-capacity screening program. This method is based on screening pools of 10 compounds. The matrix used to assemble the...