geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Effects of newly developed solutions containing trehalose on twenty-hour canine lung preservation


, : Effects of newly developed solutions containing trehalose on twenty-hour canine lung preservation. Journal of Thoracic and Cardiovascular Surgery 108(1): 92-98

Trehalose is a nonreducing disaccharide that stabilizes the cell membrane under various stressful conditions. A previous study demonstrated that trehalose was effective in 12-hour canine lung preservation. We have developed new preservation solutions containing trehalose: an extracellular type ET-Kyoto solution (Na 100 mmol/L, K 44 mmol/L) and an intracellular type IT-Kyoto solution (Na 20 mmol/L, K 130 mmol/L). The composition of these solutions is identical except for the electrolyte content. We examined their efficacy in 20-hour lung preservation. Canine lungs were flushed with ET-Kyoto (group A, n = 6), with IT-Kyoto and prostaglandin E1 (25 micrograms/kg) (group B, n = 6), or with Euro-Collins solution and prostaglandin E1 (25 micrograms/kg) (group C, n = 7), and stored for 20 hours at 4 degrees C. Left lung transplantation was performed and evaluated for up to 130 minutes. The flush time was similar in the three groups. Arterial oxygen tensions (inspired oxygen fraction = 0.5) in group A were uniformly excellent (303.3 +/- 7.0 mm Hg 70 minutes after reperfusion and 303.0 +/- 19.6 mm Hg 130 minutes after reperfusion) and significantly higher than in group B (202.6 +/- 32.0 mm Hg, p < 0.05, and 197.8 +/- 44.0 mm Hg, p = 0.054, respectively) or group C (185.9 +/- 23.0 mm Hg, p < 0.01, and 155.7 +/- 36.3 mm Hg, p < 0.05, respectively). Peak inspiratory pressure in group A was significantly lower than in groups B and C (p < 0.05). Wet/dry weight ratio in group A was significantly lower than in groups B (p < 0.05) and C (p < 0.01). Histologic and scanning electron microscopic examinations showed better preservation in group A than in groups B and C. We conclude that ET-Kyoto is superior to IT-Kyoto and to Euro-Collins solution for 20-hour lung preservation.

US$29.90

PMID: 8028386


Other references

Pask, E.A., 1955: Committee on deaths associated with anaesthesia; review of cases where post-operative care was inadequate to meet the circumstances which arose. Anaesthesia 10(1): 4-8

Zhang, B-Song., 2009: Bis(2,2'-bipyridine-κN,N')dibromido-cadmium(II). In the title complex mol-ecule, [CdBr(2)(C(10)H(8)N(2))(2)], the Cd(II) ion is six-coordinated by two cis-arranged bromide anions and four N atoms of two bidentate 2,2'-bipyridine ligands in a distorted octa-hedral geometry. The dihedral angl...

Bencsath P.; Szenasi G.; Takacs L., 1984: Role of sympathetic innervation in water and electrolyte transport of henles loop and distal tubule an in vivo microperfusion study in the rat. Acta Physiologica Hungarica 63(3-4): 219-220

Vail, G.D.; Oliver, L.R., 1993: Barnyardgrass interference in soybeans. A 2-yr field experiment was conducted at Fayetteville and Stuttgart, AR (Arizona, USA), to predict soybean yield loss as a function of barnyardgrass density and total dry weight. Predicted soybean yield reductions were 0 to 78% for original densit...

Heinze, W., 1972: Strukturanalytische Untersuchungen an den Muskeln der Vorder und Hintergliedmassen des schweines sowie Erorterung einiger allgemeinmyologischer Probleme. 3. Mitteilung: Diskussion (allgemeinmyologische Probleme, das morphologischfunktionelle System der quergestreiften Muskeln, die Bedeutung strukturanalytischer Untersuchungen fur die Zucht auf Fleischleistung), Zusammenfassung, Literatur. Anatomischer Anzeiger, 1303-4: 251-270

Maia, R.; D'Alba, L.; Shawkey, M.D., 2011: What makes a feather shine? A nanostructural basis for glossy black colours in feathers. Colours in feathers are produced by pigments or by nanostructurally organized tissues that interact with light. One of the simplest nanostructures is a single layer of keratin overlying a linearly organized layer of melanosomes that create iridesc...

Wells, R.; Van Eerd, D.; Hägg, G., 2004: Mechanical exposure concepts using force as the agent. This paper presents a model that addresses mechanical exposure with regard to the development of musculoskeletal disorders, defines exposure concepts, unifies a variety of exposures, and includes the concept of human activity. When force is used a...

Xiao, F.; Zhang, Y.; Zhang, J-ping.; Zeng, H.; Wei, H-shan., 2007: The functional effect of gamma-aminobutyric acid on hepatic stellate cells. Zhonghua Gan Zang Bing Za Zhi 15(7): 545-546

Singh, U.K.; Rajniti Prasad; Ranjeet Kumar; Jaiswal, B.P., 2006: Miltefosine in children with visceral leishmaniasis. The efficacy, tolerance, safety and drug-related adverse reactions of miltefosine in children with visceral leishmaniasis were investigated. 64 children (38 boys and 26 girls), aged 1 year to 14 years, presenting with fever, splenomegaly and posit...

Mealing, G.; Py, C., 2011: Patch-clamp array neurochips: value in interrogating simple neuronal networks with high resolution. Expert Review of Medical Devices 8(1): 3-5