geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Efficient recovery of cloned human cytomegalovirus DNA fragments from agarose gels


, : Efficient recovery of cloned human cytomegalovirus DNA fragments from agarose gels. Journal Of Virological Methods. 46(1): 1-10

A simple and efficient method for the recovery of DNA fragments from agarose gels is described. After electrophoresis, bands of interest are cut out of the gel and agarose slices pushed through the opening of a syringe needle. The resulting gel slurry is frozen and thawed three times and then centrifuged. DNA in the supernatant is precipitated, resuspended in a small volume of buffer and, finally, desalted. A recombinant pAT153 plasmid carrying the BamHI-P fragment of the human cytomegalovirus (HCMV) genome was detected using purified viral HindIII-E DNA fragment as a probe. Restriction endonuclease analysis was used to confirm the identity of the cloned fragment. Experiments performed with the recombinant pLCR127 plasmid indicate that our freeze/thaw method, with about 80% recovery and very little DNA degradation, is more advantageous than the well known electroelution and NaI/glass methods.

US$19.90

PMID: 8175942

DOI: 10.1016/0166-0934(94)90011-6


Other references

Gailing, O.; Wachter, H.; Leinemann, L.; Hosius, B.; Finkeldey, R.; Schmitt, H.P.; Heyder, J., 2003: Characterisation of different provenances of late flushing pedunculate oak (Quercus robur L.) using DNS markers from the chloroplast genome. Late flushing oaks were introduced into Germany at the end of the 19th century from the lowlands of the river Save between Zagreb and Belgrade. Trees are characterized by their late bud burst, but also by a sparely ramnified and upright stem and a...

Hester A.S., 1986: Fungi become more useful for recombinant dna products. Genetic Technology News 6(9): 9

Suzuki, O.; Nozawa, Y.; Abe, M., 2005: The regulatory roles of cell surface sialylation and N-glycans in human B cell lymphoma cell adhesion to galectin-1. Surface sialylation and glycosylation of tumor cells is known to affect various biological phenomena. In the present study, we analyzed the regulatory roles of cell surface sialylation in cell adhesion to galectin-1 in the human diffuse large B ce...

Bernard, O.; Fazekas de S.G.oth, B.; Ullrich, A.; Green, W.; Schlessinger, J., 1987: High-affinity interleukin 2 binding by an oncogenic hybrid interleukin 2-epidermal growth factor receptor molecule. Both interleukin 2 (IL-2) and epidermal growth factor (EGF) receptors exist in two forms that differ with respect to affinity for their ligand. Only the high-affinity receptors appear to be responsible for the proliferation signal delivered upon b...

Forneris L., 1976: Seasonal variation of benthic infaunal standing crop at the bay of flamengo sao paulo brazil. Anais da Academia Brasileira de Ciencias 47(SUPPL): 147

Giri, T.K.; Mazumdar, A.; Santra, S.C., 2005: Major NTFP items and their marketing potentials at Hazaribagh forest area in Jharkhand - a case study. The present study focused on the marketing aspects of selected non-timber forest products (NTFPs) of commercial importance. Emphasis has been given to NTFP items collected by the villagers/VFMPC members in and around the Hazaribagh forest in Jhark...

Lévesque, B.; Pereg, D.; Watkinson, E.; Maguire, J.S.; Bissonnette, L.; Gingras, S.; Rouja, P.; Bergeron, M.G.; Dewailly, E., 2008: Assessment of microbiological quality of drinking water from household tanks in Bermuda. Bermuda residents collect rainwater from rooftops to fulfil their freshwater needs. The objective of this study was to assess the microbiological quality of drinking water in household tanks throughout Bermuda. The tanks surveyed were selected ran...

Gaidamavichene, L.M. (Gaidamaviciene, L), 1985: Comparative evaluation of the clinical symptoms of neurotoxoplasmosis. The signs and symptoms encountered in 157 patients with chronic neurological conditions, 85 (including 78 women) of which reacted positively for Toxoplasma gondii in at least one of the 4 immunological tests used (group I) were analysed statistica...

Petersen, G.W.; Bell, J.C.; Mcsweeney, K.; Nielsen, G.A.; Robert, P.C., 1995: Geographic information systems in agronomy. Sparks, D L [Editor] Advances in Agronomy 67-111

Shabi, E., 1978: Epidemiology of pear scab in Israel. Special report: (28) 14-16