+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Elevated expression of the ornithine decarboxylase gene in human esophageal cancer

, : Elevated expression of the ornithine decarboxylase gene in human esophageal cancer. Cancer Research 52(23): 6671-6675

The mechanisms involved in sustaining the high levels of ornithine decarboxylase (ODC) activity in human cancers are not well defined. We examined the level of expression of ODC mRNA together with ODC activity in surgically excised human cancers, including esophagus, stomach, colon, and liver tumors, the objective being to determine whether the ODC mRNA level correlates with enhancement of ODC activity in these cancers. Among these tumors, the esophageal cancers had the highest ODC activity (120 +/- 43.9 pmol of CO2/h/mg of protein), compared with the stomach (37.6 +/- 13.7), colon (22.8 +/- 5.9), and liver (10.2 +/- 5.6) cancers. A remarkable increase in ODC mRNA was seen in all of the esophageal cancers. The ratio of ODC mRNA in the tumors, relative to the paired normal tissues, was 14.6 +/- 3.7. Some increase was noted in some of the stomach (2.9 +/- 0.9) and colon (2.1 +/- 0.9) cancers, but there was no increase in the liver tumors (0.9 +/- 0.2). A significant correlation was noted between ODC activity and mRNA expression in cancerous and noncancerous tissues of the esophagus, stomach, and colon, thereby suggesting that increased steady-state mRNA may be responsible for the high ODC activity in these tumors. Southern blot analysis of the DNA from the esophageal cancers revealed no amplification or significant rearrangement of the gene. Mechanisms sustaining high ODC mRNA levels in esophageal cancers may be an enhancement of the promoter activity of this gene or stabilization of the mRNA.


PMID: 1423312

Other references

Colin Alvarez, A., 1983: A comparison of the production of F1 Yorkshire X Landrace females and backcrossed females at a commercial farm in Veracruz State. For 1579 F1 and "backcross" [female][female] resp., the number of services per farrowing averaged 1.15 and 1.22, litter size at birth 9.37 and 9.47, number of liveborn piglets per litter 8.65 and 8.55, litter birth weight 12.22 and 11.93...

Brondz, B.D., 1962: Some questions of the study of the mechanisms of depression and stimulation of the growth of homografts. Uspekhi Sovremennoi Biologii 54: 207-227

Kohn, A.D.; Takeuchi, F.; Roth, R.A., 1996: Akt, a pleckstrin homology domain containing kinase, is activated primarily by phosphorylation. Akt is a serine/threonine kinase that is stimulated by receptor tyrosine kinases and contains a pleckstrin homology domain. One model proposed to explain this activation suggests that receptor tyrosine kinases stimulate a phosphatidylinositol 3-ki...

McCann, P.D., 2007: Venous thromboembolism prophylaxis: who's right--orthopedic surgeons or chest physicians?. American Journal of Orthopedics 36(9 Suppl): 2-3

Chen Shanqing, 1984: Petrographical composition of the Late Permian coal and its metamorphism in Hubei, Hunan, Guangdong and Guangxi provinces, China. Bulletin of the Yichang Institute of Geology and Mineral Resources, Chinese Academy of Geological Sciences = Yichang Dizhi Kuangchan Yanjiuso Sokan 8: 93-109

Hogan, A.M.; Vargha-Khadem, F.; Kirkham, F.J.; Baldeweg, T., 2005: Maturation of action monitoring from adolescence to adulthood: an ERP study. This study investigated the development of the frontal lobe action-monitoring system from late childhood and adolescence to early adulthood using ERP markers of error processing. Error negativity (ERN) and correct response negativity (CRN) potenti...

Shapiro R.; Kim Y.; Michael A.F.; Gerrard J.M.; Nesbit M.; Coccia P.; Ramsay N.K.C.; Krivitt W., 1979: L asparaginase induced hypo complementemia in acute lymphocytic leukemia of childhood. Serum complement [C] studies were carried out in 5 children with acute lymphocytic leukemia. During therapy with L-asparaginase, prednisone and vincristine, hypocomplementemia developed in all patients, and disappeared within 2 wk after the discon...

Plessi M.; Monzani A.; Coppini D., 1988: Determination of the monosaccharide and alcohol content of balsamic and other vinegars by enzymatic methods. The carbohydrate and alcohol contents of various types of vinegar, including traditional balsamic vinegar from Modena, "Modena Balsamic Vinegar," wine vinegar and vinegar prepared from apple must, were studied. Assays were carried out us...

Ejersted, C.; Andreassen, T.T.; Hauge, E.M.; Melsen, F.; Oxlund, H., 1995: Human parathyroid hormone increases bone strength and density of the vertebral body in old rats. Calcified Tissue International 56(5): 453

Stief, P.; Schramm, A.; Altmann, Dörte.; Beer, D., 2003: Temporal variation of nitrification rates in experimental freshwater sediments enriched with ammonia or nitrite. Two freshwater sediments (organic-poor and organic-rich) that contained their distinct natural microbial communities were incubated in experimental microcosms with either NH<sub>4</sub>+ or NO<sub>2</sub>- in the overlying...