+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Enhancement of glycine release from human brain cortex synaptosomes by acetylcholine acting at M4 muscarinic receptors

, : Enhancement of glycine release from human brain cortex synaptosomes by acetylcholine acting at M4 muscarinic receptors. Journal of Pharmacology and Experimental Therapeutics 266(1): 142-146

Synaptosomes prepared from fresh specimens of human cerebral cortex were labeled with [3H]glycine ([3H]Gly) and distributed in parallel superfusion chambers. Exposure to 15 mM KCl evoked a tritium overflow which was largely prevented by 10 mM Mg++, suggesting a consistent component of Ca(++)-dependent [3H]Gly release. Acetylcholine (ACh; 1-100 microM), added during K(+)-depolarization, increased the release of tritium in a concentration-dependent manner (maximal effect, 60%; EC50 = 7 microM). Oxotremorine (1-100 microM) mimicked ACh. The effect of 10 microM ACh was insensitive to the nicotinic antagonist mecamylamine (100 microM), but it was blocked by the muscarinic antagonist atropine (0.1 microM). Three muscarinic receptor antagonists, pirenzepine, AF-DX 116 (11-[12-[diethylamino-methyl]-1-piperidinyl]acetyl-5-11-dihydro -6H-pyrido-[2-3-b][1,4]benzodiazepine-6-one) and himbacine, endowed with relative selectivity for various muscarinic receptor subtypes, prevented with differential affinities the effect of 10 microM ACh. Himbacine was the most potent antagonist of ACh, its pA2 (8.34) being 20- or 50-fold higher than that of pirenzepine (7.27) or AF-DX 116 (6.65). It is concluded that: 1) ACh can increase the release of Gly in human cerebral cortex; 2) the interaction occurs through muscarinic receptors which resemble most the M4 subtype; and 3) considering that Gly is required to activate the N-methyl-D-aspartate glutamate receptor, the ACh-evoked Gly release may represent a linkage between cholinergic and glutamatergic transmission, two systems strongly implicated in cognitive processes.


PMID: 8392547

Other references

Schaad, N.W.; Gaush, P.; Postnikova, E., 2002: Use of portable real-time PCR for same-day on-site field diagnosis of bacterial diseases. Diagnosing bacterial diseases can be very time consuming. Traditional isolation and pathogenicity tests are very sensitive but require 10-20 days or longer. Serological techniques can reduce the time, but the detection threshold is only 104-105 cf...

Link B.; Levav I.; Cohen A., 1982: The primary medical care practitioners attitudes toward psychiatry. General practitioners [GP] are important to the delivery of mental health care because they perform 3 important functions, as an identifier, as a referral agent and as a caregiver. The importance of the attitudes G.P. hold on their performance of...

Phillips, V.L., 1997: Plug mounted prosthesis. Official Gazette of the United States Patent & Trademark Office Patents 1194(2): 1078, Jan 14

Toshchakov, R.A.; Volokitin, A.S., 1977: Endoprosthetic repair of the esophagus in inoperable cancer with the aid of a device for recanalization. Khirurgiia: 129-131

Hachaj, T.; Ogiela, M.R., 2016: The adaptation of GDL motion recognition system to sport and rehabilitation techniques analysis. The main novelty of this paper is presenting the adaptation of Gesture Description Language (GDL) methodology to sport and rehabilitation data analysis and classification. In this paper we showed that Lua language can be successfully used for adap...

Pearson, W.M., 1952: Improvement of function when structural normalization is limited. Journal of Osteopathy 59(10): 16-19

Nissen, R.; Bitschai, J., 1959: Jacob Bitschai, 1894-1958. Journal of the International College of Surgeons 31(2): 45-6; English Transl 46

Langworthy V.W., 1975: Proceedings of the 3rd annual pollution control conference of the water and waste water equipment manufacturers association industry solutions 1975 air water noise solid waste april 1 4 1975. Proceedings Of The 3rd Annual Pollution Control Conference Of The Water And Waste Water Equipment Manufacturers Association Industry Solutions Air Water Noise Solid Waste April 1-4 915

Rademacher, A., 2017: Dramatizing Deepwater Horizon. Science 356(6335): 256-256

Bornhäuser, M.; Kröger, N.; Schwerdtfeger, R.; Schafer-Eckart, K.; Sayer, H.G.; Scheid, C.; Stelljes, M.; Kienast, J.; Mundhenk, P.; Fruehauf, S.; Kiehl, M.G.; Wandt, H.; Theuser, C.; Ehninger, G.; Zander, A.R., 2005: Allogeneic haematopoietic cell transplantation for chronic myelogenous leukaemia in the era of imatinib: a retrospective multicentre study. Objective: To analyse the results of allogeneic haematopoietic cell transplantation (HCT) in patients with advanced stages of Philadelphia chromosome-positive chronic myelogenous leukaemia (CML) who had previously been treated with imatinib mesyla...