geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Evaluation of an alanine racemase gene as an indicator for the detection of various Escherichia coli: Reactivity of the gene fragment with various E. coli O157:H7 isolates


, : Evaluation of an alanine racemase gene as an indicator for the detection of various Escherichia coli: Reactivity of the gene fragment with various E. coli O157:H7 isolates. Journal of Fermentation and Bioengineering 84(5): 471-474

We determined whether various Escherichia coli O157:H7 isolates could be detected using a probe, an alanine racemase gene (dadX) fragment, specific to E. coli. One hundred strains of E. coli O157:H7 were isolated from fecal samples of healthy cattles and human patients with diarrhea and from a leg of beef. Most isolates (95 strains) produced both verocytotoxin 1 (VT1, Shiga-like toxin 1) and verocytotoxin 2 (VT2, Shiga-like toxin II), whereas four isolates produced only VT2. Although neither VT1 nor VT2 was detected in one isolate under the conditions used, the VT2 gene was detected. When all of the isolates were analyzed by a DNA hybridization method involving the use of the probe, positive signals were obtained from all isolates. The DNA hybridization method used for detection of E. coli is considered to detect various E. coli O157:H7 isolates which differ in the types of verocytotoxin they produce.

US$19.90

DOI: 10.1016/s0922-338x(97)82010-7


Other references

Lyalin, O.O.; Ktitorova, I.N.; Marichev, G.A., 1994: Polyethylene glycol ghosts of Nitella cells and the possibility of using them to study the properties of cell walls. An extremely simple method was proposed for obtaining "ghosts" of Nitella syncarpa cells. The "ghosts" consisted of plasmalemma-free cell walls and were able to retain turgor at the account of polyethylene glycol incorporated i...

Reuss, B.; Asif, A.R., 2015: Antibodies directed to the gram-negative bacterium Neisseria gonorrhoeae cross-react with the 60 kDa heat shock protein and lead to impaired neurite outgrowth in NTera2/D1 cells. Children of mothers with prenatal gonococcal infections are of increased risk to develop schizophrenic psychosis in later life. The present study hypothesizes an autoimmune mechanism for this, investigating interactions of a commercial rabbit anti...

Nola, P.; Rudan, N.; Maricić, Z.; Vukas, D., 1975: Treatment of metastatic hypernephroma with progestins. Lijecnicki Vjesnik 97(12): 687-690

Yari Khosroushahi, A.; Naderi-Manesh, H.; Toft Simonsen, H., 2011: Effect of Antioxidants and Carbohydrates in Callus Cultures of Taxus brevifolia: Evaluation of Browning, Callus Growth, Total Phenolics and Paclitaxel Production. To control the tissue browning phenomenon, callus growth, total phenolics and paclitaxel production, in the current investigation, we evaluated the effects of citric acid and ascorbic acid (as antioxidants) and glucose, fructose and sucrose in cal...

Masson, J.M., 1976: Perversions: some observations. Israel Annals of Psychiatry and Related Disciplines 14(4): 354-361

Lilly, George R., 2002: Ecopath modelling of the Newfoundland Shelf: observations on data availability within the Canadian Department of Fisheries and Oceans. University of British Columbia Fisheries Centre Research Reports 10(1): 22-27

Wilson, W.R.; Han Sen, A.E., 1936: Study of the serum lipids by a microgravimetric technique. The method is based on alcohol-ether extraction of 1 cc of serum, followed by saponification with KOH. The diieJ residue, after adding water, is acidified and extracted with petroleum ether. The extract is dried and weighed to give the unsaponifia...

Shephard, G.S.; Louw, M.E.; Labadarios, D., 1987: Analysis of vitamin B6 vitamers in plasma by cation-exchange high-performance liquid chromatography. Journal of Chromatography 416(1): 138-143

Meinert, R.; Schüz, J.; Kaletsch, U.; Kaatsch, P.; Michaelis, J., 2001: Leukemia and non-Hodgkin's lymphoma in childhood and exposure to pesticides: results of a register-based case-control study in Germany. Previous studies have suggested an association between exposure to pesticides and different types of childhood cancer. This paper presents results from a population-based case-control interview study of parents of children less than 15 years of ag...

Spencer, K.A., 1981: Agromyzidae (Diptera) in Iraq. Notes are provided on 24 species of agromyzids collected in Iraq in March 1979, 17 of them new to the country. The food-plants are recorded, many of them plants of economic importance. Several of the agromyzids are well-known agricultural pests.