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Evaluation of electrolyte leakage, chlorophyll fluorescence and ultrastructural techniques for detecting effects of acid mist on frost hardiness of sitka spruce shoots


, : Evaluation of electrolyte leakage, chlorophyll fluorescence and ultrastructural techniques for detecting effects of acid mist on frost hardiness of sitka spruce shoots. Environmental & Experimental Botany 34(3): 261-273

Two grafted clones (141, DF) of Sitka spruce (Picea sitchensis L.) were exposed to acid mist containing 1.6 and 0.1 mol m-3 concentrations of NH-4+, NO-3-, H+ and SO-4-2- at pHs 2.5 and 5.0, respectively, in open-top chambers for 27 weeks from May to November. In late November, needle and shoot samples were collected for microscopy and frost hardiness assessments. Electrolyte leakage, ultrastructural observations and chlorophyll fluorescence methods showed that clone 141 was more frost hardy than clone DF. Ultrastructural observations indicated that both clones treated with acid mist were in a more advanced state of hardening compared with controls. Chlorophyll fluorescence and electrolyte leakage data were not always in agreement, confirming that these two methods assess different physiological processes which are not equally sensitive to acid mist or freezing temperatures. Measurements of electrolyte leakage showed a significant effect of acid mist and clone on the shoots' ability to withstand sub-zero temperatures. Chlorophyll fluorescence, as assessed by the F-v/F-m ratio, measured post-freezing, showed that the activity of photosystem II is less sensitive to acid mist than the plasmalemma. The results demonstrate that the F-v/F-m ratio cannot be used as a substitute for electrolyte leakage in establishing differences in effects of acid mist on freezing injury.

US$19.90

DOI: 10.1016/0098-8472(94)90047-7


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Krause, C.; Syrovets, T.; Buechele, B.; Simmet, T., 2002: Inhibition of human monocyte activation by the pentacyclic triterpene acetyl-alpha-boswellic acid. Naunyn-Schmiedeberg's Archives of Pharmacology 365(Supplement 1): R72, March

Dudev, T.; Lin, Y-lin.; Dudev, M.; Lim, C., 2003: First-second shell interactions in metal binding sites in proteins: a PDB survey and DFT/CDM calculations. The role of the second shell in the process of metal binding and selectivity in metalloproteins has been elucidated by combining Protein Data Bank (PDB) surveys of Mg, Mn, Ca, and Zn binding sites with density functional theory/continuum dielectri...

Amoroso, L., 2016: The Second International Conference on Nutrition: Implications for Hidden Hunger. The Second International Conference on Nutrition (ICN2) was jointly organized by the Food and Agriculture Organization of the United Nations (FAO) and the World Health Organization (WHO) and was held at the FAO Headquarters in Rome, Italy, from 19...

Zhang, Y.; Wu, X-Hou.; Luo, C-Li.; Zhang, J-Mo.; He, B-Cheng.; Chen, G., 2010: Interleukin-12-anchored exosomes increase cytotoxicity of T lymphocytes by reversing the JAK/STAT pathway impaired by tumor-derived exosomes. Tumor-derived exosomes express tumor antigens, leading to their promising utility as tumor vaccines, but they also can suppress T-cell signaling molecules and reduce cytotoxic effects. We investigated whether interleukin-12 (IL-12)-anchored exosom...

Mason P., 1999: Weirs for the Cooks. NIWA hydrological expertise is helping to establish new water resources facilities in the Pacific islands.

Dharaskar, S.A.; Varma, M.N.; Shende, D.Z.; Yoo, C.Kyoo.; Wasewar, K.L., 2014: Synthesis, characterization and application of 1-butyl-3 methylimidazolium chloride as green material for extractive desulfurization of liquid fuel. The possible application of imidazolium ionic liquids as energy-efficient green material for extractive deep desulfurization of liquid fuel has been investigated. 1-Butyl-3-methylimidazolium chloride [BMIM]Cl was synthesized by nucleophilic substi...

Martin P.A.; Roberts D.J., 1989: A comparison of naked oats and barley in a silage based diet for dairy cows. Animal Production 48(3): 636

Naha, P.M., 1979: Induction of G1- cells at high frequency. Experimental conditions are reported on the induction of G1- cells at high frequency by ultraviolet irradiation of temperature-sensitive cells arrested in G1 phase at the restricted temperature. Neither the wild type cells of Balb/C-3T3 nor the te...

Luria, Salvatore, 1935: Size of nerve cells in animals whose growth has been artificially arrested. Spinal ganglion cells from hypoplastic albino rats were constantly smaller than those from normal animals of equal age, and the variability in the size of individual ganglion cells was less in hypoplastic animals than in the normal. The av. volume...

Lang, A.E., 1987: Manipulating the dopaminergic system in Parkinson's disease. Pharmacology & Therapeutics 32(1): 51-76