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Experimental identification of a scalable reactor configuration for lignin peroxidase production by Phanerochaete chrysosporium

, : Experimental identification of a scalable reactor configuration for lignin peroxidase production by Phanerochaete chrysosporium. Journal Of Biotechnology. 52(1): 21-29

The present work concerns the experimental verification of a suitable scalable reactor configuration for lignin peroxidases production by Phanerochaete chrysosporium. Tests were performed in 30 ml working volume Erlenmayer flasks, 150 ml Fernbach flasks and a 5 cm ID, 83 cm high Trickle Fixed Bed Reactor working in batch mode with 250 ml of liquid medium. Shear stress, biofilm thickness and aeration have been shown to be the key parameters for the control of lignin peroxidase production. Biofilm thickness was identified as a main scale up parameter. The results, compared with those found in the literature, are encouraging for further optimization of the reactor device and scale increase. Biofilm thickness and gas and liquid superficial velocities have been identified as variables in the optimization of lignin peroxidase production in a Trikle Fixed Bed Reactor configuration.


DOI: 10.1016/s0168-1656(96)01620-3

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