geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Expression of an allozyme of prorenin-converting enzyme in the submandibular gland of DBA/2N mice


, : Expression of an allozyme of prorenin-converting enzyme in the submandibular gland of DBA/2N mice. Journal of Biochemistry 124(2): 368-376

A protein product of the tissue kallikrein gene family was isolated from the submandibular gland of DBA/2N mice. Amino acid sequencing showed this protein to be highly homologous to two tissue kallikreins, mK13 and mK26, also known as prorenin-converting enzymes PRECE and PRECE-2, respectively. The cDNA corresponding to the present enzyme was cloned, and its complete nucleotide sequence was determined. The cloned cDNA was different in 6 and 12 bases out of 783 nucleotides from those of mK1k-13 and mK1k-26 cDNAs, respectively, the homologies being 99.2 and 98.5% (nucleotide), or 98.3 and 96.2% (amino acid). Upon incubation with either bovine kininogens or mouse Ren 2 prorenin, this tissue kallikrein generated bradykinin and renin, respectively, as judged by Western blotting and protein sequence analysis. Isoelectric focusing analysis of the submandibular gland tissue kallikreins suggested that the present enzyme was not expressed in CD-1 or ICR mice and that no mK13 protein was present in DBA/2N mice. These data suggest that the enzyme is an allozyme of mK13, a prorenin-converting enzyme highly expressed in the submandibular gland of DBA/2N mice. The mK1k-13 gene in mice is therefore suggested to be polymorphic, having at least two allelic forms with a high sequence homology. The designation mK13(b) and mK1k-13(b) for the protein and gene of this tissue kallikrein is proposed.

US$19.90

PMID: 9685728

DOI: 10.1093/oxfordjournals.jbchem.a022121


Other references

Wang, H.; Rangan, V.S.; Sung, M-Chen.; Passmore, D.; Kempe, T.; Wang, X.; Thevanayagam, L.; Pan, C.; Rao, C.; Srinivasan, M.; Zhang, Q.; Gangwar, S.; Deshpande, S.; Cardarelli, P.; Marathe, P.; Yang, Z., 2015: Pharmacokinetic characterization of BMS-936561, an anti-CD70 antibody-drug conjugate, in preclinical animal species and prediction of its pharmacokinetics in humans. CD70 is a tumor necrosis factor (TNF)-like type II integral membrane protein that is transiently expressed on activated T- and B-lymphocytes. Aberrant expression of CD70 was identified in both solid tumors and haematologic malignancies. BMS-936561...

Menar A.B., 1971: Chemical processes for phosphate removal. Water Research: 369-389

Jayasuriya, S., 1991: Technology generation and transfer for sustainable upland agriculture: problems and challenges in Southeast Asia. ACIAR proceedings series: 3) 70-76

Menetrey, J.; Kasemkijwattana, C.; Day, C.S.; Bosch, P.; Fu, F.H.; Moreland, M.S.; Huard, J., 1999: Direct-, fibroblast- and myoblast-mediated gene transfer to the anterior cruciate ligament. The anterior cruciate ligament (ACL) has poor capabilities of healing. Maturation or "ligamentization" of the ACL following autograft or allograft reconstruction has been found slow and remains under investigation. In vitro and in vivo s...

Besuchet, Claude, 1993: Coleoptera Pselaphidae of the Fangataufa atoll. Two species of Pselaphidae have been collected on the Fangataufa atoll. Lectoplectus perraulti n. sp. is described.

Vorasubin, N.; Calzada, A.P.; Ishiyama, A., 2014: Methadone-induced bilateral severe sensorineural hearing loss. Methadone, a long-acting opiate agonist, and naltrexone, an opiate receptor antagonist, are both commonly used to treat patients with morphine and heroin addiction. We present a rare case of methadone-induced persistent bilateral sensorineural hea...

Sweetman, Harvey L., 1944: Selection of woody plants as winter food by the cottontail rabbit. The selection of woody spp. of plants as food by Sylvilagus floridanus mallurus was observed during the severe winter of 1942-43 when the ground vegetation was covered with snow and ice. 100 woody spp. of plants with stems sufficiently tender to b...

Czopyk, L.; Klosowski, M.; Olko, P.; Swakon, J.; Waligorski, M.P.R.; Kajdrowicz, T.; Cuttone, G.; Cirrone, G.A.P.; D.R.sa, F., 2007: Two-dimensional dosimetry of radiotherapeutical proton beams using thermoluminescence foils. In modern radiation therapy such as intensity modulated radiation therapy or proton therapy, one is able to cover the target volume with improved dose conformation and to spare surrounding tissue with help of modern measurement techniques. Novel t...

Hirschmann, H., 1954: Unerwarteter Wiederfund tropischer Nematoden (Radopholus oryzae Thome, 1949, Panagrolaimus hygrophilus Bassen, 1940, Atylenchus decalineatus Cobb, 1913) an heimischen Sumpfpflanzen. Hirschmann describes and figures Radopholus oryzae associated with the roots of Caltha palustrii. The specimens were larger than those described by T. Goodey (1936) but morphologically almost identical. She also found Atylenchus decalineatus and P...

Mehta, L., 2005: The politics and poetics of water: the naturalisation of scarcity in Western India. This book exposes the politics behind the notion of scarcity, a taken for granted driver of international policy debates and of economic thought more generally. Through its rich history and ethnography of water in Kutch, Gujarat, India, it reveals...