geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Fine structural aspects of the anterior necrotic zone in the leg bud of the chick embryo


, : Fine structural aspects of the anterior necrotic zone in the leg bud of the chick embryo. Okajimas Folia Anatomica Japonica 69(6): 369-383

A fine structural study was undertaken on the area called the anterior necrotic zone of leg buds of the chick embryo at stages 22-29. Physiologically dying cells, which were distributed in the mesenchymal area, were characterized by increased electron density of the cytoplasm, appearance of electron lucent vacuoles and condensed chromatin pattern. Many of the dying cells seemed to be fragmented into several pieces of cell debris during the necrotic process. Two kinds of cells were observed to take up the necrotic cells; one type, large cells 15 microns or more in size and irregular in shape, which had lysosomes, extended microvillus-like cytoplasmic projections and pseudopods, took up pieces of necrotic cells by phagocytosis and stored them in acid phosphate-negative and -positive vacuoles. The other type was several microns in diameter with numerous free ribosomes and stored a small number of vacuoles containing cell debris. At stage 29, profiles of necrotic cells lying free in the mesenchymal area, and cells containing one or two vacuoles filled with cell debris decreased in number as well as large cells containing numerous vacuoles, whose contents seemed to be degraded. When pieces of the anterior portion of chick leg buds at stage 24 were organ-cultured for 3 days after glutaraldehyde-fixed mouse red blood cells were injected, the red blood cells were taken up into cells in the mesenchymal area. Formation of large cells containing numerous vacuoles stuffed with red blood cells was rare. These findings may imply that most necrotic cells in the anterior necrotic zone are disposed of by macrophages and that some are endocytosed by mesenchymal cells.

US$29.90

PMID: 8469527


Other references

Srivastava L.; Chakravarty A.K.; Kumar A.; Kumar P., 1984: Comparison of elisa enzyme linked immunosorbent assay and indirect immunofluorescence in sero epidemiology of kala azar. The specificity and sensitivity of ELISA and indirect immunofluorescence [IIF] for detection of leishmanial antibodies in kala-azar using promastigotes of Leishmania donovani, were assessed. ELISA was more sensitive than IIF, although IIF was more...

Steiner, M., 1983: Effect of alpha-tocopherol administration on platelet function in man. The effect of vitamin E administration on platelet function was evaluated in a group of normal, healthy volunteers. Platelet aggregation induced by collagen, ADP and epinephrine and platelet adhesion to collagen were measured at weekly intervals i...

von Haxthausen, F., 1978: Income tax assignment of income and expenses of a laboratory community. Deutsche Medizinische Wochenschrift 103(48): 1901-1901

Beesley, W.N., 1982: The ecological basis of parasite control: ticks and flies. Tick and fly control programmes have undergone radical review in recent years. Insecticidal control measures are now often seen as a means of reducing target populations to a level sufficiently low for final eradication by biological or ecological...

Cheung, M., 2010: Drugs used in paediatric bone and calcium disorders. Calcium and bone disorders in paediatrics are treated with a variety of drugs, many of which, although licensed for use in adults, are not so in children but are nevertheless used on the basis of accepted practice. The mainstay of drug treatment f...

Hoff, R.L.; Frenkel, J.K., 1974: Cell-mediated immunity against Besnoitia and toxoplasma in specifically and cross-immunized hamsters and in cultures. The capacity of hamster peritoneal cell populations to control viability and growth of Besnoitia and Toxoplasma organisms was assessed in vivo and in vitro. Immunized hamsters reduced the homologous organisms 100- to 10000-fold over a 5-day period...

Liu, C-Hung.; Tseng, Y-Fang.; Lai, J-I.; Chen, Y-Quan.; Wang, S-Hao.; Kao, W-Fong.; Li, L-Hua.; Chiu, Y-Hui.; How, C-Kuang.; Chang, W-Han., 2017: The changes of red blood cell viscoelasticity and sports anemia in male 24-hr ultra-marathoners. In endurance sports, stress, dehydration and release of chemical factors have been associated with red blood cell (RBC) alterations of structure and function, which may contribute to sports anemia, a well-observed phenomenon during long-distance r...

Perry, Joe N., 1998: Measures of spatial pattern for counts. SADIE (Spatial Analysis by Distance IndicEs) is a new methodology to detect and measure the degree of nonrandomness in the two-dimensional spatial patterns of populations. It applies the same principles to data in the form of maps as to data in th...

Fedtke, C.D.ichgraber, G.S.hnepf, E., 1977: Herbicide induced changes in wheat chloroplast ultrastructure and chlorophyll a. Biochemie und Physiologie der Pflanzen: 71 (4) 307-312

Nelson, S.H.; Vipond, J.; Reese, K.; McKenna, K., 1983: "Transfer trauma" as a legal argument against closing a state mental hospital. Hospital & Community Psychiatry 34(12): 1160-1162