geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Fingerprinting human chromosomes by polymerase chain reaction-mediated DNA amplification


, : Fingerprinting human chromosomes by polymerase chain reaction-mediated DNA amplification. Genomics. 14(3): 728-732

We describe here a method for DNA fingerprinting of human chromosomes of Alu-polymerase chain reaction (PCR) amplification of DNA from monochromosomal hybrids, following digestion with restriction endonucleoases. DNA digestion with restriction enzymes prior to PCR amplification reduces the total number of amplified fragments. The number and pattern of bands of PCR products observed in an electrophoretic medium are chromosome specific and provide a "fingerprint signature" for individual human chromosomes. Using this approach, we have produced fingerprints for human chromosomes 2, 5, 7, 9, and 12. The applicability of this approach to chromosome identificaiton was assessed by comparing the fingerprints obtained for two different hybrids containing chromosome 7. DNA fragments specific for the long and the short arms of human chromosome 12 had also been identified. In addition, Alu-PCR-generated DNA fragments, specific for different chromosomes, were used to probe Southern blots of a hybrid cell panel to identify human chormosomes present in hybrid cell lines. The chormosomal specificity of these probes permits the identification of intact as well as rearranged chromosomes composed of segments arising from more than one chromosome.

US$19.90

PMID: 1427900

DOI: 10.1016/S0888-7543(05)80175-2


Other references

Barnes, T.Cunlieffe, 1939: The influence of heavy water and temperature on the electrical potential of frog skin. Frog skin was tied in a small glass holder containing 2 to 5 ml. of Ringer's soln. and the potentials measured with AgCl electrodes leading to a type K potentiometer. A rapid drop in temp, of 10[degree]C. produced an avg. fall in potential of...

Kranz, W.M.; Faria, R.T. de; Miranda, G.M.; Pompeu, A.S., 1982: Evaluation of cultivation systems of beans and maize in alternate rows. In field trials in 1974-8 in the central, NE and SE regions of Parana, intercropping systems of maize and Phaseolus vulgaris were studied with (a) P. vulgaris in pure stand in the wet and dry seasons, (b) maize in pure stand followed at maturity w...

Fariello, R.G., 1988: Experimental support for the implication of oxidative stress in the genesis of parkinsonian syndromes. Functional Neurology 3(4): 407-412

Uzar, E.; Kutluhan, Süleyman.; Yurekli, V.Ali.; Ilhan, A., 2008: Topiramate-induced reversible auditory hallucination. Epileptic Disorders 10(3): 240-240

Lemmel, A.; Lowen Stadt, H.; Schossler, M., 1926: Cinemamicrographs of cell cultures with simple apparatus. Arch Exp Zellforsch 3(3): 341-344

Anonymous, 1983: Chemists-How's this for user friendly?. Environmental Science & Technology 17(12): 557a-557a

Stephens Verlin Cory; Deacon Jeffrey R., 1997: Occurrence and distribution of selected pesticides in bed sediment and fish tissue in the upper Colorado River basin, Colorado, 1995. American Water Resources Association Technical Publication Series TPS Pages 115

Woolf, A.S.; Winyard, P.J.D., 2002: Molecular mechanisms of human embryogenesis: developmental pathogenesis of renal tract malformations. The focus of this review is the normal and abnormal development of the kidney and lower urinary tract; for convenience, we will refer to the whole system as the renal tract. The content represents a convergence among the clinical disciplines of hi...

Melzer, J., 1919: Os longicorneos brazileiros da sub-familia Prioninae. Revista do Museu Paulista, 11 3-207

Cheltsov-Bebutov, AM.; Danilenko, EA.; Danilenko, AK., 1989: Moulting localities of the non-breeding common crane in northern Kazakhstan. Soobshcheniya Pribaltiiskoi Komissii po Izucheniyu Migratsii Ptits Akademiya Nauk Estonskoi SSR: 21: 151-164