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Flow cytometric enumeration and kinetic analysis of inflammatory cell populations in breast carcinomas


, : Flow cytometric enumeration and kinetic analysis of inflammatory cell populations in breast carcinomas. Analytical Cellular Pathology 5(6): 321-330

Inflammatory cell populations were quantitated in 76 consecutive mechanically dissociated clinical breast carcinoma specimens using multiparameter, two-color (PI-cytokeratin/FITC, PI-LCA/FITC) flow cytometric analysis. The percent LCA-positive events varied from 1.3-62.5% (mean = 11%) and correlated with degree of histologic inflammatory cell infiltrate (mild-5.8% LCA + events vs. marked-35.9% LCA + events, P lt 0.001), abnormal DNA content (diploid range - 4.6% LCA + events vs. aneuploid - 13.3% LCA + events, P = 0.003) and poor tumor differentiation (well-moderate - 6.5% LCA + events vs. poor - 19.9% LCA + events, P = 0.001). Synthesis phase fractions in LCA-positive populations were uniformly less than the corresponding cytokeratin (CK)-positive cells (mean LCA + SPF = 4.4% vs. mean CK + SPF = 15.5%) and varied from 3-11%. Proliferation among inflammatory populations did not correlate statistically with the total percent of LCA or CK-positive events. nor with the SPF in epithelia) populations. However, proliferative activity of inflammatory components was greater in tumors with predominately intratumoral, vs. peritumoral, inflammatory cell distribution (4.6% vs. 3.3%, P = 0.03) and in tumors with greater numbers of large, 'transformed' lymphocytes (few = 3.25% vs. many = 6.5% LCA + SPF, P = 0.001). We conclude multiparameter flow cytometric analysis of mechanically-dissociated breast tumors is representative of tumor infiltrating lymphoid populations in breast tumors and provides a potentially useful means of studying biologically relevant tumor-host interactions.

US$29.90

PMID: 8305328


Other references

Nagato, Yuko; Kawaguchi, Shigeo, 2003: Seasonal changes of Sargassum fusiforme at Shikanoshima on the northern coast of Kyushu, Japan, according to the fluctuations in weight of main branches. Seasonal changes in the vegetative morphology and reproductive state of Sargassum fusiforme (Harvey) Setchell were investigated over a 10-month period at Shikanoshima on the northern coast of Kyushu, Japan. The population was sampled at nearly mon...

Hodgson, J.C.; Mellor, D.J.; Field, A.C., 1982: Foetal and maternal rates of urea production and disposal in well-nourished and undernourished sheep. Five monotocous and 2 ditocous Scottish Blackface ewes with indwelling catheters in an umbilical artery and vein of 1 fetus only and in both maternal jugular veins were used. Experiments were conducted over a period of 2 d [days] when [14C]urea wa...

Kellogg, J.A.; Atria, P.V.; Sanders, J.C.; Eyster, M.E., 2001: Intra- and interlaboratory variabilities of results obtained with the Quantiplex human immunodeficiency virus type 1 RNA bDNA assay, version 3.0. Normal assay variation associated with bDNA tests for human immunodeficiency virus type 1 (HIV-1) RNA performed at two laboratories with different levels of test experience was investigated. Two 5-ml aliquots of blood in EDTA tubes were collected...

Sirianni M.C.; Testi R.; Bonomo G.; Aiuti F.; Morrone A., 1984: Lack of specific cell mediated immunity to cytomegalovirus in people at risk for acquired immunodeficiency syndrome. Twenty apparently healthy homosexuals from Italy and 5 patients with lymphoadenopathic syndrome (LAS) were studied for specific cell mediated immunity (CMI) to cytomegalovirus (CMV), by means of the leukocyte migration inhibition test (LMIT). Tota...

Hua, H.Bao., 2008: The market in concubines in Jiangnan during Ming-Qing China. The traffic in concubines flourished in Ming-Qing Jiangnan as a result of large scale interactions of political and socioeconomic contexts. How women were situated within the system is central to this study, which revolves around two major argumen...

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Duddridge M.; Walters E.H., 1990: Prophylactic anti inflammatory treatment of asthma variations throughout the world. O'byrne, P M (Ed ) Allergic Disease And Therapy, Vol 2 Asthma As An Inflammatory Disease Ix+320p Marcel Dekker, Inc : New York, New York, Usa; Basel, Switzerland Illus 283-312

Szucs, E.; Acs, I.; Csiba, A.; Ugry, K., 1995: The role of group size in developing housing management of dairy cows II Ethological aspect. In a 2 times 2 factorial experiment using lactating Holstein-Friesian and Hungaro-Friesian cows the effect of group size (15-80 head) was investigated on behaviour parameters of animals. Expt. 1 was conducted in cow sheds with deep litter serving...

Weiland, G.; Rommel, M.; von Seyerl, F., 1979: Serological cross-reactions between toxoplasma and hammondia. Toxoplasma and Hammondia infected mice, dogs, rabbits, and pigs were tested for Toxoplasma antibodies by means of 5 serological methods. All Toxoplasma infected animals showed Toxoplasma-specific antibodies. Only sera of Hammondia infected mice an...

Ulrich R.G.; Atassi M.Z., 1989: Alloreactive t cell recognition of the hla dr beta amino terminal polymorphic region. T cell alloreactivity, originally discovered as a tissue transplantation effect, is believed to be a manifestation of the normal major histocompatibility complex (MHC) restriction of antigen presentation by accessory cells to T cells. The molecula...