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Functional domains of human RAP74 including a masked polymerase binding domain

, : Functional domains of human RAP74 including a masked polymerase binding domain. Journal of Biological Chemistry 270(45): 27035-27044

RAP74, the large subunit of human transcription factor IIF (TFIIF), has been analyzed by deletion mutagenesis and in vitro assays to map functional domains. Tight binding to the RAP30 subunit involves amino acids between positions 1-172. Amino acids 1-205 are minimally sufficient to stimulate accurate transcription from the adenovirus major late promoter in an extract system, although C-terminal sequences contribute to activity. A partially masked RNA polymerase II binding domain has been mapped to the C-terminal region of the protein (amino acids 363-444). Sequences near the N terminus and within the central portion of RAP74 affect accessibility of this domain. Extending this domain to 363-486 creates a peptide that binds polymerase and DNA and inhibits transcription initiation in vitro from non-promoter DNA sites. This larger C-terminal domain may modify polymerase interaction with template during initiation and/or elongation of RNA chains.


PMID: 7592953

DOI: 10.1074/jbc.270.45.27035

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Constant P.; Renou A.M.; Vernhiet J.; Caille J.M., 1977: Cerebral ischemia studied by computed tomography. Journal of Computer Assisted Tomography 1(3): 359-360

Crawford, J.A.; Oates, R.M.; Helfer, D.H., 1979: Avian pox in California quail from Oregon. California quail (Lophortyx californicus) from several locations in Oregon were examined seasonally between fall 1975 and summer 1978 for avian pox. Pox lesions were present on 66 of 256 (26%) birds from the E.E. Wilson Wildlife Area. None of the...

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Aronson R.B.; Van Slyke D.D., 1971: Manometric determination of carbon di oxide combined with scintillation counting of carbon 14. Analytical Biochemistry 41(4): 173-188

Coll, J.; Geraud, G.; Arne, M.C.; Bes, A., 1981: Hyperthermic nystagmographic tests: first results in multiple sclerosis. Journal Francais D'oto-Rhino-Laryngologie; Audiophonologie, Chirurgie Maxillo-Faciale 30(6): 373-375

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Flint, O.Jr, 1974: Studies of Neotropical caddisflies. XVII. The genus S micridea from North and Central America (Trichoptera: H ydropsychidae). Smithsonian contributions to zoology: 67 65

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