geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Genome fingerprinting of the silkworm, Bombyx mori, using random arbitrary primers


, : Genome fingerprinting of the silkworm, Bombyx mori, using random arbitrary primers. Electrophoresis. 16(9): 1633-1638

The random amplified polymorphic DNA (RAPD) technique was used to study DNA profiling of thirteen silkworm genotypes. The genotypes included six diapausing and seven nondiapausing varieties that represent a high degree of divergence with respect to geographic origin, and morphological, qualitative, quantitative and biochemical characters. Two hundred sixteen amplified products were generated using 40 random primers. Genotype-specific amplification products were identified. Amplification products specific to diapausing genotypes were also identified. Segregation of the RAPD marker was analyzed in a backcross population and found to be inherited as dominant Mendelian traits. Based on pairwise comparison of amplified products, the genetic similarity coefficient was calculated using the NeiLi similarity coefficient, and cluster analysis was performed by a hierarchical clustering technique. Silkworm genotypes were clustered into two groups, one consisting of six diapausing and the other of seven nondiapausing genotypes. The results of our study suggest that the RAPD technique could be used as a powerful tool to generate genetic markers that are linked to traits of interest in the silkworm.

US$19.90

PMID: 8582347

DOI: 10.1002/elps.11501601270


Other references

Yanagawa, T.; Yamamoto, N.; Suzuki, A., 1978: The modification of the responses to noradrenaline and acetylcholine by aging and the effects of some smooth muscle relaxants on the smooth muscle contractile drugs. Experiment in the isolated vas deferens of SHRSP and Wistar-Kyoto rats. Japanese Heart Journal 19(4): 642-643

Blatz P.; Mohler J.H.; Ahmed W., 1990: Spectroscopic observation of solvent interaction with selected retinal schiff bases and implications of rhodopsin. Photochemistry & Photobiology 51(SUPPL): 92S

Stone, W.E.; Boyden, B.L.; Wisecup, C.B.; Tatman, E.C., 1932: Control of the Celery Leaf-tier in Florida. During winters of high temperature in Florida considerable damage is done to celery by Phlyctaenia rubigalis, Guen. To some extent injury can be avoided by early harvesting, as the most mature plants are preferred for oviposition. Directly after h...

Peters, A., F.; Ramirez, M.-Eliana, 2001: Molecular phylogeny of small brown algae, with special reference to the systematic position of Caepidium antarcticum (Adenocystaceae, Ectocarpales). Nuclear ribosomal DNA (3'-SSU, ITS, 5'-LSU) and plastid-encoded (rbcL and Rubisco spacer) sequences were determined in Caepidium antarcticum and compared to homologous sequences of relatives from Ectocarpales, Scytothamnales, and other b...

Rampini M.; Zoia S., 1990: A new cave bathysciinae from sardinia patriziella nuragica new species coleoptera cholevidae. Patriziella nuragica n. sp., a new species of Bathysciinae from North-West Sardinia (Italy); is described and illustrated. Several morphological characters clearly differentiate it from P. sardoa Jeannel, 1956: smaller size, different shape of pro...

Woodford J.A.T.; Lerman P.M., 1974: Morphological variation in spring migrants of myzus persicae hemiptera aphididae comparison of alatae from peach and mangold. Bulletin of Entomological Research 64(4): 595-604

Sharma, V.K.; Agarwal, D.S.; Satyanand; Saha, K., 1980: Study of immune status of patients with severe burns. IgM, IgG, IgA, IgD and IgE were measured in blister-fluids and sera of 12 patients with extensive burns at the time of admission and thereafter in sera collected on the 3rd day and subsequently at weekly intervals for 1 mo. Severely burnt patients...

L.N.nsheng; L.H.ngsheng; Ding Dewen; Wan Shuxia, 2003: A singular perturbation method for determining phase change temperature in a petroleum pipeline in seasonally frozen ground for irregular boundary configuration. Two-dimensional phase change temperature in petroleum pipelines in seasonally frozen ground regions under severe climate conditions was studied. The singular perturbation method was used to solve the unsteady phase change temperature question. Per...

NM.J.; RM.R.; G.G.; Maliakel, B.; D.S.; IM.K., 2016: Beyond the flavour: a de-flavoured polyphenol rich extract of clove buds (Syzygium aromaticum L) as a novel dietary antioxidant ingredient. Though kitchen spices constitute an important source of dietary antioxidants, their consumption at a physiologically relevant dose is very often hampered by their unpleasant flavour characteristics. The present paper describes a novel approach to...

Löf, M.; Henriksson, H.; Forsum, E., 2013: Evaluations of Actiheart, IDEEA® and RT3 monitors for estimating activity energy expenditure in free-living women. Activity energy expenditure (AEE) during free-living conditions can be assessed using devices based on different principles. To make proper comparisons of different devices' capacities to assess AEE, they should be evaluated in the same popul...