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Genomic characterization of type 3 polioviruses isolated from vaccine-associated poliomyelitis cases in Brazil

, : Genomic characterization of type 3 polioviruses isolated from vaccine-associated poliomyelitis cases in Brazil. Brazilian Journal of Medical and Biological Research 28(2): 195-200

Eight strains of P3/Sabin-related polioviruses were analyzed; four from persistent paralytic poliomyelitis cases classified as vaccine associated, one from a transient paralysis case classified as transverse myelitis, one from a transient paralysis case classified as Guillain-Barré syndrome, one from a transient facial paralysis case, and one from a healthy vaccine. The serotypes of the viral isolates were identified by the neutralization test with hyperimmune equine sera and the relationship of the isolates with the P3/Sabin strain was demonstrated by molecular hybridization of the viral RNA of the isolates with a P3/Sabin-specific probe. The P3/Sabin relationship was confirmed by PCR, using a pair of specific primers for P3/Sabin-related isolates. The available data indicate that a U-->C mutation at nucleotide 472 in the 5' noncoding region of the genome of the type 3 Sabin strain increases the neurovirulence of this strain and this mutation was observed in all type 3 isolates from vaccine-associated cases. These eight P3/Sabin-related isolates were partially sequenced in the 5' noncoding region and seven presented a U-->C mutation at nucleotide 472, except the isolate from a transient paralysis case classified as transverse myelitis, that maintained a U at nucleotide 472. Although this virus maintaining U at nucleotide 472 may not be the etiological agent of the disease, the possibility that the virus was the causative agent of the disease could not be ruled out.


PMID: 7581041

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Bin ImJung; Hun PakHwa, 1995: Study on the penetrating quantity suitable for the rice culture in the paddy field of fine sand tideland. The soil infiltration capacity suitable for the paddy rice cultivation in a fine sandy soil is 4-9 mm/day. To ensure a suitable soil water regime for rice production the water depth should be controlled in relation to the infiltration capacity of...

Dhir, V.; Fort, M.; Mahmood, A.; Higbee, R.; Warren, W.; Narayanan, P.; Wittman, V., 2012: A predictive biomimetic model of cytokine release induced by TGN1412 and other therapeutic monoclonal antibodies. Human peripheral blood mononuclear cells (PBMC) are routinely used in vitro to detect cytokine secretion as part of preclinical screens to delineate agonistic and antagonistic action of therapeutic monoclonal antibodies (mAbs). Preclinical value o...

Schneider, W., 1986: Designation des Lectotypus von Somatochlora meridionalis Nielsen 1935 (Odonata: Anisoptera: Corduliidae). Entomologische Zeitschrift, 966: 73-78

Neri, I.; Piccolo, V.; Russo, T.; Bianchi, A.; Patrizi, A., 2013: Congenital primarily ulcerated hemangioma mimicking cleft lip. Journal of Pediatrics 162(4): 882-882.E1

Shneider, B.L., 1998: A new era in bile acid transport pathophysiology. Journal of Pediatric Gastroenterology and Nutrition 26(2): 236-237

Anonymous, 2011: Prepuberal evolution of plasma leptin levels in gilts ofthrifty genotypeIberian pig and lean commercial crosses Large White × Landrace. Current study aimed to determine possible differences in plasma leptin levels during the prepuberal period and their relationship with the onset of puberty in gilts of obesethrifty genotype(Iberian breed) and lean genotype (Large White Landrace co...

de Pinho, M.B.; Tavano, O.; Lopes, E.S., 1987: Analysis of variations of exposure and development times of Kodak (Ektaspeed) and Agfa-Gevaert (Dentus M-2) periapical films. Revista de Odontologia Da Universidade de Sao Paulo 1(1): 21-30

Fedosova N.U.; Boldyrev A.A., 1991: A nonhyperbolic kinetics of sodium potassium atpase a new viewpoints. The kinetics of the 130 kDa monomer obtained by treatment of duck salt gland Na,K-ATPase with C12E8 was compared with that of the membrane-bound enzyme. The shapes of the substrate-velocity curves for the membrane-bound and solubilized forms were...

Kauppi, P.H.ri, P., 1980: Packaging materials significance for plant care during the transport and storage of planting material. Arsskrift Norske skogplanteskoler: ub 1980) 51-55

Macner, M.; Solecka-Derkacz, W., 1985: Visceral lupus erythematosus with fulminant course complicated by massive gastrointestinal hemorrhage. Wiadomosci Lekarskie 38(2): 131-133