+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Glucocorticoid induction of epinephrine synthesizing enzyme in rat skeletal muscle and insulin resistance

, : Glucocorticoid induction of epinephrine synthesizing enzyme in rat skeletal muscle and insulin resistance. Journal of Clinical Investigation 92(1): 303-307

Rat skeletal muscle contains two enzymes which can make epinephrine: phenylethanolamine N-methyltransferase (PNMT) and nonspecific N-methyltransferase. We studied the time-course and mechanism by which the glucocorticoid dexamethasone increases muscle PNMT activity. We also examined the hypothesis that increased muscle E synthesis may contribute to glucocorticoid-induced insulin resistance. Dexamethasone (1 mg/kg s.c. for 12 d) increased muscle PNMT activity seven-fold but did not change NMT activity. Immunotitration with an anti-PNMT antibody indicated that the PNMT elevation was due to increased numbers of PNMT molecules. Dexamethasone rapidly increased PNMT activity and this elevation was largely maintained 6 d after glucocorticoid treatment stopped. Muscle epinephrine levels were transiently elevated by dexamethasone. Dexamethasone-treated rats had elevated insulin levels after a glucose load, and chronic administration of the PNMT inhibitor SKF 64139 reversed this increase. Chronic SKF 64139 improved glucose tolerance in normal rats. Dexamethasone induced muscle synthesis of the epinephrine-forming enzyme PNMT. A PNMT inhibitor lowered insulin levels in glucocorticoid-treated rats and glucose levels in untreated rats. These findings are compatible with antagonism of insulin-mediated glucose uptake by epinephrine synthesized in skeletal muscle.


PMID: 8325998

DOI: 10.1172/JCI116567

Other references

Rahmann, H.; Esser, M., 1965: Bestimmung der Sehscharfe Minimum separable sowie Dressurverhalten des skandinavischen Berglemmings Lemmus lemmus, L. Zeitschrift fuer Saugietierkunde, 30: 47-53

Warren Harry Verney; Delevault Robert E., 1948: Biogeochemical investigations in British Columbia. Biogeochemistry, like geophysics, is a useful tool for those who seek ore in areas where outcrops are few, vegetation heavy, or glacial drift widespread.Knowledge of the normal amounts of, as well as the ratios existing between, any elements in th...

Simpson, I., 2002: Inpatient group work for patients with psychosis. Nurses working on acute inpatient wards usually think in terms of individualised patient care. Indeed, this ethos is at the heart of modern nursing. This article suggests that a different perspective is needed if we are to recognise the place of t...

Nakatani, M.; Kawashima, S., 1994: An attempt to assess leaf color of winter cereals using a simple video system. The estimation of the chlorophyll content of leaves of wheat, barley and rye was attempted using an 8 mm video camera and a personal computer system. First, we took video images of a color chart for rice leaf from various directions outdoors, and...

Koskas, R.; Zirah, P., 1961: A new anti-staphylococcal agent of value: triacetyloleandomycin. Results of the treatment of various staphylococcias by triacetyloleandomycin in hospital milieu. La Tunisie Medicale 39: 615-626

Falzarano, M.; Pinto Zipp, G., 2013: Seeking consensus through the use of the Delphi technique in health sciences research. The purposes of this paper are to explain how the Delphi technique has been used as a research methodology for seeking consensus among experts in the health science literature and to offer a model for its future use. The authors discuss the propos...

Engel, T., 2002: Good initiative. Ugeskrift for Laeger 163(46): 6451-6451

Prince, T.L.; Helman, S.W.; Leffell, M.S.; Martin, W.H.; Moores, R.R.; Stafford, C.T.; Wray, B.B., 1994: Chronic herpes simplex infection, natural killer dysfunction and human papilloma virus infection associated with myelodysplasia. Journal of Allergy & Clinical Immunology 93(1 PART 2): 276

Nielen, M.; Schukken, Y.H.; Brand, A.; Haring, S.; Ferwerda-van Zonneveld, R.T., 1995: Comparison of analysis techniques for on-line detection of clinical mastitis. Three techniques were compared for analysis of automatically collected data from the milking parlor. Mammary quarters showing signs of clinical mastitis were compared with randomly selected healthy quarters. Automatic data were analyzed from the m...

Sutherland, S.D.; Vickery, R.K.Jr, 1993: On the relative importance of floral color, shape, and nectar rewards in attracting pollinators to Mimulus. Pollinator preferences were observed for the six species of section Erythranthe of the genus Mimulus using greenhouse-grown plants placed in a meadow in the Red Butte Canyon Natural Area, Salt Lake County, Utah. The principal pollinators were humm...