+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Glutathione-S-transferase induces murine dermatitis that resembles human atopic dermatitis

, : Glutathione-S-transferase induces murine dermatitis that resembles human atopic dermatitis. Clinical & Experimental Allergy. 26(11): 1329-1337

Background: The molecular and functional basis of allergen-induced inflammation seen in atopic dermatitis (AD) remains undefined. Objective: The objective of this study is to establish a murine model to dissect the pathological mechanisms of inflammatory reactions leading to the development of AD. Methods: An inbred strain of mice, BALB/c, when injected peritoneally with 30 Mg of recombinant Sj26 protein (rSj26), a glutathione S-transferase of Schistosoma japonicum worm, developed systematic dermatitis 21 days after immunization. The pathology of the dermatitis was examined by histological evaluation and immunostaining. The immediate skin hypersensitivity was demonstrated by serum transfer and skin test. Epicutaneous patch test was used to demonstrate the antigen-specific late phase response. Results: Significant responses of rSj26-specific IgE were detected 2 weeks after immunization, and such changes paralleled formation of skin lesions. The diseased skin pathology showed inflammatory changes such as infiltration of mononuclear cells and eosinophils in the dermis and mild spongiosis in the epidermis. Numerous IgE bearing cells were also detected in the dermis. Peripheral blood showed eosinophilia at the same time. In addition, rSj26-specific positive skin test and epicutaneous patch test could be demonstrated in rSj26-sensitized mice. Conclusions: These results suggest that rSj26 is capable of eliciting atopic dermatitis-like lesions in BALB/c mice. This can be a useful animal model for elucidating allergen-induced immune responses and the development of various therapeutic interventions of atopic dermatitis in humans.


PMID: 8955582

DOI: 10.1111/j.1365-2222.1996.tb00530.x

Other references

Dargenio R.; Iaconinni L.; Lamanna M.A.; Corbucci M.G.; Garcea N., 1990: Empty follicle the ghostbusters syndrome. Human Reproduction (Oxford) 5(SUPPL): 81

Woloszyn, M.; Trzesinski, A.; Ellis, I., 2010: Sun-protective behaviours of beach goers in the North-West. The study investigates the sun-protective behaviours of beach goers in the north-west of Western Australia as the basis for a new health promotion intervention to be implemented in the area. A cross-sectional, observational survey of 1,498 beach g...

Ortiz, G.A.; Koch, S.; Forteza, A.; Romano, J., 2008: Ramsay hunt syndrome followed by multifocal vasculopathy and posterior circulation strokes. Neurology 70(13): 1049-1051

Zhang, Y.; Jiang, B.; Li, W.; Zhou, C.; Ji, F.; Xie, Q.; Sun, X.; An, L.; Bao, Y., 2010: Mechanisms of analgesic action of Gln49-PLA(2) from Gloydius ussurensis snake venom. Gln49-PLA<sub>2</sub> is a phospholipase A<sub>2</sub> isolated from the <i>Gloydius ussurensis</i> snake venom. In this paper, we studied its effect on the function of neural conduction. Electrophysiological st...

Singh, M.; Lennon, R.J.; Gulati, R.; Holmes, D.R., 2014: Risk scores for 30-day mortality after percutaneous coronary intervention: new insights into causes and risk of death. To determine the causes and risk of death after percutaneous coronary interventions (PCIs) and to compare the discriminatory ability of the New York State Risk Score (NYSRS) with the Mayo Clinic Risk Score (MCRS). We studied in-hospital and 30-day...

Heros, D.O.; Renkens, K.; Kasdon, D.L.; Adelman, L.S., 1988: Patterns of recurrence in glioma patients after interstitial irradiation and chemotherapy: report of three cases. Three patients with malignant gliomas who developed dissemination of tumor at the time of recurrence are presented. All three patients received combination therapy including conventional radiotherapy and interstitial radiation therapy followed by...

Takahashi, Y.; Chihara, K., 1998: Clinical significance and molecular mechanisms of bioinactive growth hormone (review). About 80% of short children are not deficient in endogenous growth hormone (GH) and termed idiopathic short stature (ISS). The causes of impaired growth in children with ISS are various. Short stature and low insulin-like growth factor-I (IGF-I) c...

Ljustina, S.; Argirovic, R.; Tosic, V.; Kadija, S., 2016: Sigmoid-vaginal fistula as a rare complication following vaginal surgery. International Urogynecology Journal: -

Rocha, N. da C., 2003: Effects of acetazolamide use on the eggs shell formation in hens. This study was conducted to determine the effects of acetazolamide on egg shell formation in laying hens. 40 hens were individually kept in cages and subdivided into 4 groups (I, II, III, and IV). Group I served as control, whereas the other 3 gro...

Larenas Linnemann, Désirée.; Rodríguez Pérez, N.; Becerril, Mín., 2008: Adverse reactions to skin tests and immunotherapy in the practice of Mexican allergologists. Immunotherapy is the only curative treatment recommended for allergic rhinoconjunctivitis and asthma, in which small amounts of allergen are administered sublingually or subcutaneously until the maximum tolerated dose has been reached. However, lo...