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Growth of fish cell lines in glutamine-free media

, : Growth of fish cell lines in glutamine-free media. Cytotechnology. 16(3): 159-166

The glutamine requirement for the in vitro proliferation of fish cells was investigated with cell lines from four different species and three tissues: goldfish skin (GFSK-S1), Chinook salmon embryo (CHSE-214), and rainbow trout liver (RTL-W1) and spleen (RTSp-W1). With a supplement of fetal bovine serum, the basal medium, Leibovitz's L-15, without glutamine supported the proliferation of all four cell lines as well, or nearly as well, as L-15 with 2 mM glutamine. This was true over short term assays of two to four weeks and for continuous propagation. CHSE-214 also grew as well with or without 2 mM glutamine in Minimum Essential Medium with fetal bovine serum. However, when the supplement was dialyzed fetal bovine serum, CHSE-214 grew much better in L-15 without glutamine. Therefore, glutamine was not required for growth in L-15, and in fact, was inhibitory in the absence of the dialyzable fraction of serum. By contrast, glutamine appeared to be important for growth in Minimum Essential Medium. When the supplement was dialyzed fetal bovine serum, CHSE-214 grew much better in Minimum Essential Medium with 2 mM glutamine. These results suggest that the glutamine requirement for the in vitro proliferation of fish cells is conditional and depends on the basal medium and serum supplement.


PMID: 7766144

DOI: 10.1007/bf00749903

Other references

Foley, J.M.; Berman, R.J.; Smith, C.E., 1960: X-ray irradiation of Coccidioides immitis arthrospores: survival curves and avirulent mutants isolated. At Naval biol. Lab., Sch. Public Health, Univ. Calif., Berkeley, suspensions of arthrospores of C. immitis, containing uninucleate and binucleate spores in a 1: 1 ratio, were subjected to 1 kiloroentgen (kr.) approx./min. for varying time periods....

Gupta, G.N.; Prasad, K.G.; Mohan, S.; George, M., 1988: Effect of soil texture and rainfall on stocking and growth of naturally occurring tree species. Soil profile and vegetation studies were carried out in 366 soil pedons and quadrats in Coimbatore Forest Division, Tamil Nadu. The data collected included soil texture, rainfall, numbers of trees/ha for different species, and their girth, density...

Wilson, C.M.; Bulleid, N.J., 2003: Investigation of folding and degradation of mutant proteins synthesized in semipermeabilized cells. Methods in Molecular Biology 232: 295-312

Miró, O.; López, S.; Casademont, J.; Cardellach, F., 2001: Mitochondrial involvement in antiretroviral therapy-related lipodystrophy of HIV-infected patients. Medicina Clinica 117(18): 716-716

Dunsmore, J.R., 1988: Mountain environmental management in the Arun river basin of Nepal. The study considers environmental management within the southern part of the Arun river basin which lies in Dhankuta, Bhojpur and Sankhuwasabha Districts, eastern Nepal. The study area is divided into two zones. The northern zone lies above 2500 m...

Tallón Lobo, S.; Bueno, C.; Becerra, A.; Jociles, J.; Pérez-Miranda, M., 1991: Empty sella turcica associated with diabetes insipidus. A report of a new case. Revista Clinica Espanola 189(1): 45-45

Zdichynec, B.; Roberts, V.A., 1974: Unusual case of metastasis of Grawitz tumor in the lower jaw recognized with one year latency. Ceskoslovenska Stomatologie 74(6): 439-443

Keleher, J.J., 1961: Comparison of largest Great Slave Lake fish with North American records. The largest sizes for 11 of 25 species of Great Slave Lake fish are presented from material collected during studies dating from 1944 and other sources. The North American size records compiled from a literature search also are given. Only two of...

Lindblom, U.F.; Ottosson, J.O., 1956: Bulbar influence on spinal cord dorsum potentials and ventral root reflexes. Acta Physiologica Scandinavica 35(3-4): 203-214

Krishnan, J.A.; Diette, G.B.; Rand, C.S., 2001: Disparities in outcomes from chronic disease. Bmj 323(7319): 950-950