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High frequency of K-ras mutations in normal appearing lung tissues and sputum of patients with lung cancer

, : High frequency of K-ras mutations in normal appearing lung tissues and sputum of patients with lung cancer. International Journal Of Cancer. 63(6): 810-814

To evaluate the possible use of mutant ras as a biomarker for lung cancer, we have analyzed "normal appearing" lung tissue, lung tumor, lung metastases and sputum samples from patients with non-small cell lung cancer (NSCLC). As a control, we used lung tissue and sputum samples from patients without oncological diseases or lung disorders. Our analyses were performed with the aid of enriched PCR (EPCR), a method which enables detection of ras mutation even if present at low incidence. EPCR identified K-ras codon 12 mutations in 10% of lung tissues obtained from patients with no lung diseases, whereas the same mutation was detected in 60% of samples of normal appearing lung tissues obtained from patients with NSCLC, 62% of NSCLC tumors and 80% of metastases. Analysis of sputum samples of patients with NSCLC identified 47% to harbor mutant ras allele, whereas 12.S% of controls diagnosed with non-oncological lung diseases carried this mutation. Most of these mutations were detected with the aid of EPCR only, indicating that a minority of cells in a given sample harbor this mutation. The ability to detect K-ras codon 12 mutation in 60% of lung tissue samples and in 47% of sputum samples taken from patients with lung cancer (as compared with 10% and 12.5% of respective controls) points to the potential use of ras mutation as a biomarker for exposure and possible identification of patients who may be at a higher risk of developing lung cancer.


PMID: 8847139

DOI: 10.1002/ijc.2910630611

Other references

Riedl, O., 1977: Ideologically-political orientation of scientific information in medicine. Casopis Lekaru Ceskych 116(18): 545-547

Gargiulo, A.R.; Hill, J.A., 1999: Autoimmune endocrinopathies in female reproductive dysfunction. Volpe, R [Editor] Contemporary Endocrinology; Autoimmune endocrinopathies 365-391

Delp, H.A., 1951: Goals for the mentally retarded. American Journal of Mental Deficiency 55(4): 472-478

Pick, F., 1950: Inclusion des oeufs d'Ascaris megalocephala a l'aide de la technique de silico-gel sur lame. A. megalocephala eggs mounted aseptically in silica gel and sealed with paraffin remained unchanged. When they were removed from the mounts after 46 days at 33[degree]C or at room temp., they developed normally. After being mounted for 2 years, th...

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Mordovets, A.A.; Chernyshev, I.D.; Nazarenko, G.I.; Golovin, V.V., 1974: Testing Tordon. Intensive harrowing, standard herbicides and earthing up control most weeds in maize except Russian knapweed [Centaurea repens] for which 3 hand weedings are required. Trials were carried out in 1966-70 on solonetsic chestnut soils in an arid clim...

Williams, J.A.; Yanik, G.A.; Hutchinson, R.J.; Levine, J.E., 2003: Autologous bone marrow transplantation for relapsed hepatoblastoma. Pediatric Research 53(4 Part 2): 302A-303A, April

Ciulla, U.; Luraschi, C., 1953: The fibrinolytic power of the blood in labor and in the puerperium. Annali di Ostetricia E Ginecologia 75(9): 1377-1394

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Lohleit M.; Baechmann K., 1990: Integrated analysis of solid samples by on line supercritical fluid extraction gas chromatography. A system for direct coupling of supercritical fluid extraction with carbon dioxide and capillary gas chromatography (GC) has been developed. Use of a double-oven gas chromatograph allows precise thermostating of all parts used for the extration an...