+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Homogeneity in the distribution of matrix components in the hamster zona pellucida as revealed by backscattered electron imaging fracture-label

, : Homogeneity in the distribution of matrix components in the hamster zona pellucida as revealed by backscattered electron imaging fracture-label. Anatomical Record 239(1): 35-46

Background: Backscattered electron imaging fracture-label (BEI-FL), an adaptation of the fracture-label method for scanning electron microscopy, offers the advantage of providing information about the distribution of antigenic and receptor sites with respect to the three-dimensional organization of tissues and cells over relatively large surfaces. Recently, using post-embedding cytochemistry on thin-sections of Lowicryl-embedded oocytes, a homogenous distribution of glycoproteins in the zona pellucida (ZP) was demonstrated (Kan et al., 1989. Biol. Reprod., 40:585-598, Anat. Rec., 226.37-47; Roux and Kan, 1991. Anat. Rec., 230:347-360). However, it can be argued that the chemical nature of resins and the physical conditions of tissue processing required for post-embedding cytochemistry may introduce changes in the tissue components and result in altered distribution of components. On the other hand, freeze-fracture exposes constituents in a minimally denaturing manner and, since no embedding media are used, binding sites are sterically available to the probe. We have, therefore, applied BEI-FL to examine the distribution of matrix glycoproteins in the ZP of hamster oocytes. Methods: Ovaries and cumulus masses obtained from superovulated female golden hamsters were fixed by immersion in 2.5% glutaraldehyde and processed for fracture-label. Tissues were labeled, respectively, with Wheat germ agglutinin (WGA) followed by ovomucoid-colloidal gold, Ricinus communis agglutinin I (RCA I)-colloidal gold or a monoclonal antibody against Hamster Oviductin-1 followed by protein A-gold, and then examined in the scanning electron microscope. Results: Backscattered electron imaging revealed a homogenous distribution of WGA and RCA I binding sites throughout the cross-fractured matrix of the ZP of ovarian and postovulatory oocytes. Hamster Oviductin-1, an oviductal glycoprotein which is transferred to the ZP of oocytes during oviductal transit, was also found to be uniformly distributed throughout the ZP of postovulatory oocytes. Conclusions: Our results indicate that BEI-FL can be advantageously used to examine extracellular matrices and are consistent with the concept that glycoproteins are uniformly distributed throughout the ZP of the hamster oocyte.


PMID: 8037376

DOI: 10.1002/ar.1092390105

Other references

Griffaton, G.; Faudemay, F.; Rozen, R.; Naon, R.; Lowy, R., 1975: Energy metabolism in the liver of rats fed a diet contaminated with dithiocarbamates. Young male rats, Wistar CF strain, weighing about 100 g, were fed during 14 days with a well-balanced diet, but containing either 275 p.p.m. nabame, either 600 p.p.m. thirame or 3 600 p.p.m. zinebe. The animals given the non-contaminated diet were...

Cooke, M.S.; Lunec, J.; Evans, M.D., 2002: Progress in the analysis of urinary oxidative DNA damage. Oxidative DNA damage has been implicated to be important in the pathogenesis of many diseases, including cancer and heart disease. The assessment of damage in various biological matrices, such as DNA, serum, and urine, is vital to understanding th...

Turner, 1874: An Illustration of the Relations of the Convolutions of the Human Cerebrum to the Outer Surface of the Skull. Journal of Anatomy and Physiology 8(Pt 2): 359-361

Tarhonskaya, H.; Chowdhury, R.; Leung, I.K.H.; Loik, N.D.; McCullagh, J.S.O.; Claridge, T.D.W.; Schofield, C.J.; Flashman, E., 2014: Investigating the contribution of the active site environment to the slow reaction of hypoxia-inducible factor prolyl hydroxylase domain 2 with oxygen. The prolyl hydroxylase domain proteins (PHDs) catalyse the post-translational hydroxylation of the hypoxia-inducible factor (HIF), a modification that regulates the hypoxic response in humans. The PHDs are Fe(II)/2-oxoglutarate (2OG) oxygenases; t...

Mitrovic, M.N.kolic, M.O.asanovic, D., 1998: Selection of walnuts. Acta Horticulturae 484: 203-205

Hunt, K., 1984: Do they finally have the guns to kill fee-for-service?. Medical Economics 61(7): 145-152

Kube, J.; Probst, S., 1999: Birds breeding in reedbeds at the southern Baltic Sea coast: What effect does reed harvesting have on population densities?. Densities of breeding bird populations were determined in 1998 in brackish water reedbeds on the island of Ruegen, German Baltic Sea coast. In order to determine potential effects of reed harvesting, we measured various parameters of the reed vege...

Dvorak, J.A.; Hyde, T.P., 1973: Trypanosoma cruzi: interaction with vertebrate cells in vitro. 1. Individual interactions at the cellular and subcellular levels. Experimental Parasitology 34(2): 268-283

Patro G.K.; Mishra M., 1986: Parlakhemundi breed of buffaloes. A study with 1,178 Parlakhemundi buffaloes in their native tract (Ganjam and Koraput districts of Orissa [India]) revealed that they were of swamp type, and were classified as Monda (large), Deshi (medium) and Jirangi (small) with different phenot...

Coburn, J.W.; Brickman, A.S.; Sherrard, D.J.; Singer, F.R.; Wong, E.G.; Baylink, D.J.; Norman, A.W., 1977: Use of 1,25(OH)2-vitamin D3 to separate 'types' on renal osteodystrophy. Patients with severe symptomatic renal osteodystrophy were treated with either 1,25(OH)2D3 or 1 alpha(OH)D3. In 39 instances, there was either reversal of symptoms and/or a marked fall in plasma alkaline phosphatase. Bone biopsies showed improveme...