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Identification of clastogenic and/or aneugenic events during the preneoplastic stages of experimental rat hepatocarcinogenesis by fluorescence in situ hybridization

, : Identification of clastogenic and/or aneugenic events during the preneoplastic stages of experimental rat hepatocarcinogenesis by fluorescence in situ hybridization. Carcinogenesis 16(8): 1825-1834

A growing body of evidence from human and animal cancer cytogenetics studies indicates that aneuploidy is an important chromosome change in carcinogenesis. To understand the role of this genetic phenomenon during the first steps of an experimental cancer model, molecular and cellular techniques were combined. A sequential cytogenetic study of a modified Solt-Farber liver cancer model in the rat was performed to identify the importance of chromosome versus genome mutations. Male Wistar rats were initiated with diethylnitrosamine (DENA), followed by a 2-acetylaminofluorene exposure to select resistant hepatocytes. Chronic phenobarbital (PB) treatment was used to induce promotion. Cell proliferation was induced by a necrogenic dose of CCl4, administered during the selection period (Gerlans protocol) or 3 days before hepatocyte isolation (experimental protocol). In order to discriminate between genetic events causing chromosome breakage (clastogenic) and those that induce chromosome loss (aneugenic), isolated micronucleated hepatocytes (MNH) were analysed for the presence of a centromere in the micronucleus (MN). Non-radioactive in situ hybridization with a rat centromere satellite 1 DNA probe was applied. Our results show that the majority of the observed genetic changes, expressed as MN during different preneoplastic stages, were of clastogenic origin. However, the number of induced aneugenic hepatocytes increased markedly during the promotion period of the Gerlans protocol (approximately 7-fold above control) and during PB exposure in the experimental protocol (approximately 4-fold above control). Additionally, these stages were also characterized by an increased level of MN expression (20.3 < % MNH < 32.8), in comparison with the initiation stage after DENA exposure (13.5 < % MNH < 17.1). Although it is not yet clear if these genetic alterations have a causative nature in neoplastic liver transformation, the use of interphase cytogenetics certainly might lead to a better understanding of the genomic changes which occur during experimental hepatocarcinogenesis.


PMID: 7634410

DOI: 10.1093/carcin/16.8.1825

Other references

Mukamal, L.V.; Ferreira, A.F.; Jacques,; Jacques,; Amorim, C.A.F.L.; Pineiro-Maceira, J.; Ramos-e-Silva, M., 2013: Cowden syndrome: review and report of a case of late diagnosis. International Journal of Dermatology 51(12): 1494-1499

Gilardi, G.L., 1973: Nonfermentative gram negative bacteria encountered in clinical specimens. Antonie van Leeuwenhoek 39(2): 229-242

Moges, A.D.; Admassu, B.; Belew, D.; Yesuf, M.; Njuguna, J.; Kyalo, M.; Ghimire, S.R., 2016: Development of Microsatellite Markers and Analysis of Genetic Diversity and Population Structure of Colletotrichum gloeosporioides from Ethiopia. Twenty three polymorphic microsatellite markers were developed for citrus plant pathogenic fungus, Colletotrichum gloeosporioides, and were used to analyze genetic diversity and population structure of 163 isolates from four different geographical...

Bahr Karsten; Simpson Fiona, 2002: Heterogeneity of the transition zone. Eos, Transactions, American Geophysical Union 83(47, Suppl

Jungk, A.; Kramarovsky, E.; Merkel, D.; Papenhagen, A.; Wehrmann, J., 1972: Effects of iron on some physiological properties of tomato plants. To determine the effects of Fe deficiency on metabolic processes, plants of cv. Eurocross B were grown in nutrient solutions with Fe concentrations of 0 to 1 p.p.m. Yield increased as Fe concentrations rose from 0.01 to 0.3, and the chlorophyll co...

Marello, L.; Barnaba, C.; Vuan, A.; Palmieri, F.; Romanelli, M.; Priolo, E.; Braitenberg, C., 2008: Gravity, seismic and ambient noise to detect the buried shape of an alpine valley. The importance of local geological conditions is a key parameter to understand the ground motion during earthquakes. In particular, in sediment-filled basins numerical simulations have indicated that 2D and 3D site effects are often important. In...

Sarhan M.A.; Mostafa M.K.; Mortada N.S., 1986: Effect of soybean flour and improvers on bread quality. Sedimentation value, fermentation time, height, volume and relative volume of bread decreased with the increase in soybean flour percentage added in flour mixtures. Addition of potassium bromate up to 18 ppm to flour mixtures containing 3% soybean...

Álvaro Ospina; Daniel Campuzano; Elizabeth Hincapié; Luisa F.V.squez; Esperanza Montoya; Isabel C.Z.pata; Manuela Gómez; José Bareño, 2012: Efficacy of facet block in lumbar facet joint syndrome patients. Facet block is a procedure used in patients with facet arthrosis in which several other medical techniques have failed. In our country, there is no evidence or studies regarding its efficacy, thus the interest in its demonstration. A retrospective...

Jürgens, P., 1988: Taurine--recent views and importance for artificial feeding. Taurine is an important age- and compartment-specific part of the pool of free amino acids in all human tissues and fluids examined. Besides an active taurine transport system, the mechanisms to assure compartment-specific taurine-homeostasis in h...

Shipley, A.E.; Macbride, E.W., 1901: Phylum Porifera, in Zoology, an Elementary Text-Book. Unknown