+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification of goat and mouse urinary metabolites of the pneumotoxin, 3-methylindole

, : Identification of goat and mouse urinary metabolites of the pneumotoxin, 3-methylindole. Xenobiotica; the Fate of Foreign Compounds in Biological Systems 23(9): 1025-1044

1. Urine from goats dosed i.v. with 3-methylindole (3MI; 15 mg/kg) or [methyl-14C] 3MI (15 mg/kg, 0.5 microCi/kg) contained at least 11 metabolites of 3MI. 2. Goat metabolized 3MI to sulfate conjugates of 4- or 7-hydroxy-3-methyloxindole, 5- or 6-hydroxy-3-methyloxindole, and 3,5- or 6-dihydroxy-3-methyloxindole; glucuronic acid conjugates of indole-3-carboxylic acid and 4- or 7-hydroxy-3-methyloxindole; and unconjugated 3-hydroxy-3-methyloxindole. Diastereoisomeric glucuronic acid conjugates of 3-hydroxy-3-methyloxindole were also identified in goat urine. 3. Urine from mice dosed i.p. with 3MI (400 mg/kg) or [ring-UL-14C] 3MI (400 mg/kg, 125 microCi/kg) contained at least six metabolites of 3MI. 4. Mice metabolized 3MI to glucuronic acid conjugates of 3,5- or 6-dihydroxy-3-methyloxindole, 5- or 6-hydroxy-3-methyloxindole, and indole-3-carboxylic acid; and unconjugated indole-3-carboxylic acid. Unconjugated 3-hydroxy-3-methyloxindole was identified in mouse urine in a previous report. 5. Both goats and mice metabolized 3MI to a mercapturate, 3-[(N-acetyl-L-cystine-S-yl)methyl]indole, which has been previously identified and was confirmed in this study. 6. 3-Methyloxindole was not identified in the urine of either goats or mice. 7. The major pathways of 3MI biotransformation in goats and mice is the formation of mono- and dihydroxy-3-methyloxindoles and their subsequent conjugation with glucuronic acid or sulfate. 8. There are no apparent qualitative differences in the biotransformation of 3MI between goats and mice that can account for their different sensitivities to 3MI-induced lung injury.


PMID: 8291262

DOI: 10.3109/00498259309057041

Other references

Schnermann, J., 2011: Maintained tubuloglomerular feedback responses during acute inhibition of P2 purinergic receptors in mice. Tubuloglomerular feedback (TGF), the change of afferent arteriolar resistance initiated by changes of luminal NaCl concentration, is thought to be related to NaCl-dependent release of ATP by macula densa cells. In the present study, we have explor...

Moomjy, M.; Colombero, L.T.; Veeck, L.L.; Rosenwaks, Z.; Palermo, G.D., 1999: Sperm integrity is critical for normal mitotic division and early embryonic development. The human zygote relies on the paternal gamete to provide the centrosome component essential for the first mitotic division. It is not known whether normal centrosome function requires an intact spermatozoon, or whether donation of an isolated pat...

Rembrink, K.; Niebel, W.; Behrendt, H., 1993: Renal autotransplantation: Indications and results. Renal autotransplantation is an established but rarely used therapy in cases of renal vessel lesions, tumours of the kidney and ureter, long-distance ureter lesions, complex nephrolithiasis and retroperitoneal fibrosis. The indications and results...

Iuriatin, A.A., 2013: Effective methods of losing weight in obesity. World experience. The prevalence of obesity among adults in Russia is 18,4-29,8%. It is a significant risk factor for a number of serious diseases. An appropriate diet and aerobic physical activity are effective in overweight management. In studies, Glucerna helped...

Lecaillon, A., 1906: Sur la structure de la couche chitineuse tegumentaire et sur les insertions musculaires de la larve de Tabanus quatuornotatus Meigen. C-R Ass anat 8e session (Bordeaux): 68-70

Burgess K.H., 1990: Floral display and oviposition site selection a mechanism for host range expansion. Bulletin of the Ecological Society of America 71(2 SUPPL): 108

Qiu, J.; He, X.; Cui, H.; Zhang, C.; Zhang, H.; Dang, Y.; Han, X.; Chen, Y.; Tang, Z.; Zhang, H.; Bai, H.; Xu, R.; Zhu, D.; Lin, X.; Lv, L.; Xu, X.; Lin, R.; Yao, T.; Su, J.; Liu, X.; Wang, W.; Wang, Y.; Ma, B.; Liu, S.; Huang, H.; Lerro, C.; Zhao, N.; Liang, J.; Ma, S.; Ehrenkranz, R.A.; Liu, Q.; Zhang, Y., 2014: Passive smoking and preterm birth in urban China. Studies investigating the relationship between maternal passive smoking and the risk of preterm birth have reached inconsistent conclusions. A birth cohort study that included 10,095 nonsmoking women who delivered a singleton live birth was carrie...

Narod, S.A., 2016: Response to Evans et al. Clinical Genetics 89(3): 400-400

England, E.P., 1985: Lasers: issues, problems, and implications for practice. Perioperative Nursing Quarterly 1(2): 29-38

Khodadadi, D.; Rousso, M.; Nacache, G.N.; Rosencwajg, J.; Mihich, M., 1980: Importance of immediate heparin therapy and systematic "second look" follow-up operations during treatment for mesenteric vein thrombosis. Report of three cases. Annales de Chirurgie 34(1): 33-35