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Immobilization of high-affinity heparin oligosaccharides to radiofrequency plasma-modified polyethylene


, : Immobilization of high-affinity heparin oligosaccharides to radiofrequency plasma-modified polyethylene. Journal of Biomedical Materials Research 27(6): 811-819

Oligosaccharides of heparin with high affinity for antithrombin III (ATIII) have been immobilized onto surface-modified NHLBI Primary Reference low density polyethylene (PE). PE was modified by radiofrequency plasma polymerized ( lt 150 nm thick) films derived from N-vinyl-2-pyrrolidone (PPNVP) or allyl alcohol (PPAA), and coupled by chemical derivatization to either 3-aminopropyltriethoxysilane or amino-terminated poly(ethylene oxide). High affinity heparin oligosaccharides (HA-heparin), anti-factor Xa activity of 592 +- 120 IU/mg) prepared by partial deaminative cleavage of commercial crude heparin and fractionated by agarose-ATIII affinity chromatography, were immobilized to surface-modified PE by reductive amination. The anticoagulant activity, as determined by a chromogenic assay for the inhibition of factor Xa, was estimated to be 30-70 mIU/cm-2, with binding estimated to be 56-119 ng/cm-2. The highest activity was obtained for the HA-heparin immobilized to PE modified by PPNVP with a PEO spacer. Visual confirmation of ATIII binding to immobilized HA-heparin was demonstrated by a gold-labeled double antibody method with imaging by SEM.

US$19.90

PMID: 8408111

DOI: 10.1002/jbm.820270614


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