+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Immunogold localization of the DnaK heat shock protein in Escherichia coli cells

, : Immunogold localization of the DnaK heat shock protein in Escherichia coli cells. Journal of General Microbiology 139(1): 95-99

Previously reported cell fractionation experiments have yielded conflicting information on the cellular localization of the DnaK heat shock protein of Escherichia coli. Here we used immunogold labelling of ultra-thin sections to determine the localization of DnaK in unstressed cells at 30 degrees C as well as in heat-shocked cells. In cells grown at 30 degrees C, gold particles were found predominantly in the cytoplasm, indicating that the majority of the DnaK molecules are cytoplasmic; however, a fraction of the gold particles was located in proximity to the membranes, raising the possibility that a subpopulation of DnaK proteins is membrane-associated. Heat shock of the cells did not induce detectable relocalization of DnaK.


PMID: 8450312

DOI: 10.1099/00221287-139-1-95

Other references

Kumar R.; Shultz T.D.; Bollman S., 1982: Cortisol diminishes intestinal calcium transport in vivo but does not alter brush border membrane vesicle calcium uptake. Federation Proceedings 41(3): ABSTRACT 58

Malachias, M.V.B.; Koch, V.; Colombo, C.; Silva, S.; Guimarães, I.C.B.; Nogueira, P.K., 2017: 7th Brazilian Guideline of Arterial Hypertension: Chapter 10 - Hypertension in Children and Adolescents. Arquivos Brasileiros de Cardiologia 107(3 Suppl 3): 53-63

Majoor, F., 1990: Early bird study weekend.. Amoeba, 645: 82-83

Zhang, W-Dong.; Han, Y-Peng.; Zong, Z-Hong.; Guo, J.; Li, M-Jiang.; Wang, X-Hui., 2010: Zoledronic acid blocks cell cycle and induces apoptosis in lung cancer cell line 95D cells and their mechanisms of action. To study the effect of zoledronic acid on cell cycle blocking and induction of apoptosis in lung cancer cell line 95D cells, and their mechanisms of action. The effect of zoledronic acid (ZOL) on proliferation of lung cancer cell line 95D cells wa...

Colli, A.M.T.; Takaki, M., 1992: Studies on spore germination in Thelypteris dentata (Forssk) E. St. John. T. dentata spores were germinated at temperatures ranging from 10 to 35 degrees C. The effects of white and far-red light were also determined. Germination was highest (>90%) at 25 degrees . The need for continuous white light irradiation, and...

Grimm, R., 1982: Mechanized tending of quickset hedges. Boundary hedges established on banks between agricultural fields in Schleswig-Holstein and managed as coppice forest are briefly described, and various coppicing methods are compared, including: (a) manual; or using a cutting tool attached to a cr...

Krimbas, Costas B., 2001: In defense of neo-Darwinism: Popper's Darwinism as a metaphysical programme revisited. Unknown

Davis, C.A., 1894: Earth Worms. Science 23(580): 150-151

Celedon, J.M.; Cline, K., 2013: Intra-plastid protein trafficking: how plant cells adapted prokaryotic mechanisms to the eukaryotic condition. Protein trafficking and localization in plastids involves a complex interplay between ancient (prokaryotic) and novel (eukaryotic) translocases and targeting machineries. During evolution, ancient systems acquired new functions and novel transloca...

Adjidé, C.C.; Bouteille, B.; Josse, R.; Adjidé-Szmidt, V.; Avodé, D.G.; Dumas, M., 1996: Seroprevalence of cysticercosis in the lacustrian community of Vekky, Atlantic district (Benin). Cysticercosis is a parasitic disease due to the infection of man with Cysticercus cellulose, the larva of Taenia solium. This disease is frequent in countries with low socio-economic development and is linked to sanitary conditions. The aim is to...