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Immunohistochemical analysis of the expression of ras oncogenes in human renal cell carcinomas

, : Immunohistochemical analysis of the expression of ras oncogenes in human renal cell carcinomas. Acta Histochemica et Cytochemica 25(5): 591-599

Fifty-one cases of human renal cell carcinoma were investigated for immunohistochemical localization of ras oncogenes and their products. The immunohistochemical results were evaluated comparatively with the histological grade, stage and progression of the tumor. The ras p21 oncogene product was positive mainly in the plasma membrane of the renal cell carcinomas. The tissues from normal kidneys were limitedly stained in the cytoplasm of the proximal convoluted tubule cells. Five of 28 (17.9%) frozen specimens of renal cell carcinoma revealed the positive staining for ras mRNA by means of in situ hybridization as well as the strongly positive staining for ras p21 gene products. These 5 cases were all grade 2 and pT2b in TNM classification. However, there was no point mutation on exon 1 (corresponding to codons 12 and 13) and exon 2 (corresponding to codon 61), where point mutations are reported in several human tumors. Positive staining cases for ras p21 (survival rate of 81.2% at 5 years) showed the tendency to decrease the survival rate compared to the ras p21 negative staining cases. There was a significant correlation of ras p21 positive staining and the pathological extent of the primary tumor (pT) (p lt 0.05). Our immunohistochemical study revealed that the expression of ras p21 is one of the parameters related to the prognosis of renal cell carcinomas.


DOI: 10.1267/ahc.25.591

Other references

Tron, E.; Brizard, A.; Sadoun, A.; Giraud, C.; Randriamalala, E.; Delwail, V.; Dreyfus, B.; Alcalay, D.; Guilhot, F., 1994: Flow cytometry guidelines for blood CD34+ concentration One center experience. Experimental Hematology (Charlottesville) 22(8): 820

Taş, H.; Şenocak, R.; Kaymak, Şahin.; Lapsekili, E., 2016: Experiences of Conflict Zone-Related Ballistic Renal Injury. To analyze our approaches and experiences in patients with renal injuries accompanying penetrating abdominal injuries admitted to the hospital due to high kinetic energy weapons. Patients including renal injuries associated with penetrating abdomi...

Wanjura, H.J., 1969: Theory and practice of artificial pacemakers. Deutsches Medizinisches Journal 20(8): 270-272

Breitenstein, K.; Buss,, K.L.ttau, G., 1974: Results of using heifers in various rearing and suckling time variants. Tierzucht 28 (3) 110-112

Lundberg A., 1968: Differential chloride reversal of inhibitory post synaptic potentials from group ia afferents and motor axon collaterals cat. Acta Physiologica Scandinavica2: 3a-4a

Barberis, C., 1967: Agriculture and rural society in Tuscan regional planning. Agricultural planning is not concerned with only one sector of economic activity, but relates to a given environment, a type of society called rural society. It is not sufficient merely to determine how much labour is technically necessary for agr...

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