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Immunohistochemical analysis of the microanatomy of primate ovary


, : Immunohistochemical analysis of the microanatomy of primate ovary. Biology of Reproduction 54(3): 734-742

The ovary is a complex organ composed of cells of diverse lineages. Therefore, in this study we examined whether immunolocalization of various cytoskeletal, epithelial, immune-cell, and neural-associated proteins can differentiate various cells in the baboon and human ovaries. Surface epithelial cells exhibited immunoreactivity for cytokeratin and desmin, however, they did not immunostain for other epithelial markers such as carcinoembryonic antigen or epithelial membrane antigen. Smooth muscle actin was distributed apically whereas vimentin was localized basally in these cells. Ova exhibited strong immunoreactivity for S-100, Leu-M1, and neurofilament and did not show immunoreactivity for epithelial and cytoskeletal proteins. In antral follicles and theca cells, and after formation of corpus luteum, both granulosa and theca cells expressed immunoreactivity for vimentin. Cytokeratins were absent in the preantral and antral follicles. However, atresia and development of apoptosis was associated with expression of immunoreactive cytokeratins in atretic follicles. Development of corpus luteum led to major changes in the immunophenotype of follicular cells. The mere presence of immunoreactivity for cytokeratin and a strong immunoreactivity for desmin in the luteinized granulosa and not in the theca cells allowed discrimination of these cells from each other and from their ancestral cells. Proliferating cell nuclear antigen was present in various ovarian cells except for ovum. The distinct patterns of expression of cytoskeletal, epithelial, and neural-associated proteins in various cells of the ovary facilitates their identification and discrimination.

US$19.90

PMID: 8835398

DOI: 10.1095/biolreprod54.3.734


Other references

Zabelina, M.V., 2005: Content of free amino acids in a protein of a muscular tissue of young growth of sheeps. In age aspect the author investigated a muscular tissue of young growth of sheeps of different breeds on the presence in it of free amino acids and correlation between essential and nonessential amino acids. It was shown, that the raised level of...

Popham, F.; Walker, J.J.; Mitchell, R., 2007: Could using general health and longstanding limiting illness as a joint health outcome add to understanding in social inequalities research?. Journal of Public Health 29(2): 208-210

Van de Velde, S.; Roex, A.; Vangronsveld, K.; Niezink, L.; Van Praet, K.; Heselmans, A.; Donceel, P.; Vandekerckhove, P.; Ramaekers, D.; Aertgeerts, B., 2013: Can training improve laypersons helping behaviour in first aid? A randomised controlled deception trial. There is limited evidence indicating that laypersons trained in first aid provide better help, but do not help more often than untrained laypersons. This study investigated the effect of conventional first aid training versus conventional training...

Fuller R.W.; Taylor G.W.; O'connor B.J., 1992: Cysteinyl leukotriene production in asthma effects of mast cell and neurally mediated bronchoconstriction. American Review of Respiratory Disease 145(4 PART 2): A290

Leone, M.; Franzini, A.; D'Amico, D.; Grazzi, L.; Mea, E.; Curone, M.; Tullo, V.; Broggi, G.; D'Andrea, G.; Bussone, G., 2004: Strategies for the treatment of autonomic trigeminal cephalalgias. Trigeminal autonomic cephalalgias (TACs) are a group of primary headache syndromes characterised by two main clinical characteristics: pain and oculofacial autonomic phenomena. Three headache forms are grouped as TACs: cluster headache (CH), parox...

Arreola, J.; Melvin, J.E.; Begenisich, T., 1995: Volume-activated chloride channels in rat parotid acinar cells. 1. Rat parotid acinar cells undergo a regulatory volume decrease in response to hypotonically induced cell swelling that is sensitive to K+ and Cl- gradients. To investigate the potential mechanisms involved, the whole-cell patch-clamp technique w...

Delvalle, J.A.; Greengard, O., 1976: The regulation of phenylalanine hydroxylase in rat tissues in vivo. The maintenance of high plasma phenylalanine concentrations in suckling rats: a model for phenylketonuria. Maximum inhibition of phenylalanine hydroxylase activity in the liver (85%) and in the kidney (50%) of suckling rats required the administration of over 9 mumol of p-chlorophenylalanine/10g body weight. Despite the decrease in the total activity f...

Xin, Y.; Lasker, J., M.; Lieber, C., S., 1995: Serum carbohydrate-deficient transferrin: Mechanism of increase after chronic alcohol intake. Carbohydrate-deficient transferrin (CDT) is now considered to be the most sensitive and specific biological marker of alcohol abuse. However, the mechanism by which chronic alcohol consumption causes an elevation of CDT levels in serum is still no...

Barthel F., 1978: State and development of uranium prospecting methods; an overview. Schriftenreihe der GDMB Gesellschaft Deutscher Metallhuetten-und Bergleute

Ciani, B.; Jourdan, M.; Searle, M.S., 2004: Stabilization of beta-hairpin peptides by salt bridges: role of preorganization in the energetic contribution of weak interactions. A model beta-hairpin peptide has been used to investigate the context-dependent contribution of cross-strand Lys-Glu interactions to hairpin stability. We have mutated two Ser-Lys interstrand pairs to Glu-Lys salt bridges, one close to the type I&...