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Immunohistochemical analysis of the relation between 5-hydroxytryptamine- and neuropeptide-immunoreactive elements in the spinal cord of an amphibian (Xenopus laevis)


, : Immunohistochemical analysis of the relation between 5-hydroxytryptamine- and neuropeptide-immunoreactive elements in the spinal cord of an amphibian (Xenopus laevis). Journal of Comparative Neurology 341(4): 492-506

In mammals, a large proportion of the bulbospinal 5-hydroxytryptamine (5-HT) neurons also contain neuropeptides, such as substance P (SP) and galanin (GAL). To examine whether a similar coexistence occurs in an amphibian, an immunofluorescence double-labelling technique was employed on sections of the Xenopus laevis spinal cord. Antisera raised against SP, GAL, enkephalin (ENK), corticotropin-releasing factor (CRF), calcitonin gene-related peptide (CGRP), and cholecystokinin (CCK) produced a labelling of fibers at all rostrocaudal levels of the spinal cord, with the highest fiber densities for SP and ENK and intermediate densities for GAL, CCK, and CGRP, while CRF-immunoreactive fibers were barely detectable in intact animals. 5-HT-immunoreactive fibers were widely distributed in the spinal cord, and they often occurred in the vicinity of different types of peptide-immunoreactive fibers. However, no coexistence between 5-HT and the different peptide immunoreactivities could be detected, although SP and GAL immunoreactivities were sometimes found to be colocalized in the same fiber. Similar negative results were obtained when 5-HT + SP- and 5-HT + GAL-labelled sections were examined in single focal planes with a confocal microscope. After a spinal transection, (survival period 6 weeks to 4 months), almost all 5-HT-immunoreactive fibers below the lesion were lost, and a build-up of immunoreactive material occurred in fibers just rostral to the cut. In contrast, no significant loss of peptide-immunoreactive fibers occurred, although some swollen SP-, GAL-, ENK-, CRF-, and CCK-immunoreactive fibers were present rostral to the cut. The distribution of swollen peptide-immunoreactive fibers did not overlap with that of the swollen 5-HT-immunoreactive fibers. Although negative immunohistochemical data must be interpreted with caution, in conjunction with previous studies (Brodin et al. (1988) J. Comp. Neurol. 271:1-18; Sakamoto and Atsumi (1991) Cell Tissue Res. 264:221-230), the present results indicate that bulbospinal 5-HT neurons in nonmammalian vertebrates cocontain neuropeptides to a lesser extent than in mammals.

US$19.90

PMID: 7515401

DOI: 10.1002/cne.903410406


Other references

Gong J.K., 1971: X ray induced myelogenous leukemia in rats. Radiation Research 47(1): 250

Beetens, J.R.; Van Den Bossche, R.; Herman, A.G., 1982: Production of 6-Oxo-PGF1 alpha by aorta rings in the presence of ascorbic acid. Archives Internationales de Pharmacodynamie et de Therapie 256(1): 151-152

Hertz, J.; Larsen, P.; Pedersen, L.M., 1978: Diabetogenic effects of ritodrine. Severe hyperglycemia and ketoacidosis in 5 pregnant diabetics and 1 nondiabetic during treatment with ritodrine or ritodrine combined with betamethasone. Ugeskrift for Laeger 140(5): 223-226

Woledge, R.C., 1999: Muscle energetics during unfused tetanic contractions. Modelling the effects of series elasticity. During an unfused tetanic contraction the contractile component stretches and then is stretched by the series elasticity in the muscle fibre during each tension oscillations. This causes the heat rate to increase, from increased metabolic rate, du...

Schultze-Motel, W., 1970: A monograph of the moss genus Andreaea. I. The costate species. Willdenowia (1) 25-110

Cahn, M.M.; Levy, E.J.; Shaffer, B., 1956: Polymorphous light eruption; the effect of chloroquine phosphate in modifying reactions to ultra-violet light. Journal of Investigative Dermatology 26(3): 201-207

Stiehm, E.R., 1992: The psychologic fallout from Chernobyl. American Journal of Diseases of Children 146(6): 761-762

Godshalk, C.P., 1994: Common pitfalls in radiographic interpretation of the thorax. Examples of normal variations in radiographs of the thorax mistakenly believed to represent disease are presented. These include dorsal and rightward deviation of the cranial thoracic trachea, cardiomegaly versus obesity, pleural "fluid"...

Dashdeleg N.; Romanov N.N.; Shcheglova O.P., 1982: Possible use of aerospace data for pluvial flood forecasting in Mongolia. Pages 55-61:

Grimm, Roland., 2001: Faunistics and taxonomy of some species of the genus Tribolium Macleay, 1825, with a description of three new species from Africa Coleoptera, Tenebrionidae Faunistik und Taxonomie einiger Arten der Gattung Tribolium Macleay, 1825, mit Beschreibung von drei neuen Arten aus Afrika Coleoptera, Tenebrionidae. Entomofauna. 31 Dezember; 2219: 393-404